Antigen: Aggregatibacter actinomycetemcomitans serotype d
Available to For-Profits: Yes
Antigen Species: Aggregatibacter actinomycetemcomitans
Hybridoma Cells Available (Non-Profit): No
Depositor: Gmuer, R.
Host Species: mouse
Positive Tested Species Reactivity: Aggregatibacter actinomycetemcomitans serotype d
Depositors Institution: University of Zuerich, Institute of Oral Biology, ZZMK
Antigen Molecular Weight:
Immunogen: Aggregatibacter actinomycetemcomitans, strains OMZ 542 (= IDH 781), IDH 328
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Myeloma Strain: SP2/0-Ag14
Epitope Mapped: No
Antibody Registry ID: AB_2618065
Recommended Applications: Immunofluorescence, Western Blot
Additional Information: *Cell lines will be distributed only with the written consent of University of Zurich's tech transfer office, Ref. UZ-18/273. Contact the DSHB for details (email@example.com).
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
330AA3.2 was deposited to the DSHB by Gmuer, R. (DSHB Hybridoma Product 330AA3.2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).