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SKU: 3D3-s

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DSHB Data Sheet

Catalog Fields

Product Name/ID: 3D3
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: Dnm1
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_2631263 
Uniprot ID: P21575 
Entrez Gene ID: 140694 
Clonality: Monoclonal
Immunogen: Phosphorylated peptide
Immunogen Sequence: RASPSRPES857PRPPC
Myeloma Strain: SP/0
Epitope Mapped: Yes
Antigen Name: dynamin-1xa, phosphorylated
Epitope Location or Sequence: dynamin 1 amino acids 849-861
Alternate Antigen Name:
Deposit Date: 3/1/2017
Antigen Molecular Weight: 97.2 kDa
Depositor: Hwang, Yu-wen
Antigen Sequence:
Depositor Institution: City University of New York
Antigen Species: Rat
Depositor Notes: Liu Y, Adayev T and Hwang YW. An ELISA DYRK1A non-radioactive assay suitable for the characterization of inhibitors [version 1; referees: 2 approved]. F1000Research 2017, 6:42 (doi: 10.12688/f1000research.10582.1
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Human, Mouse, Rat
Additional Information: Dynamin 1 is phosphorylated by DYRK1A, a serine/threonine kinase.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization: https://f1000research.com/articles/6-42/v1 
Recommended Applications: ELISA, Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3D3 was deposited to the DSHB by Hwang, Yu-wen (DSHB Hybridoma Product 3D3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

2 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication
    IF References

    Activity-dependent phosphorylation of dynamin 1 at serine 857.
    Hwang YW
    Biochemistry 51.34 (2012 Aug 28): 6786-96.

    WB References
    All References

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