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SKU: 3D3-s
View product citations for antibody 3D3 on CiteAb

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Available: 28

DSHB Data Sheet

Catalog Fields

Antigen: dynamin-1xa, phosphorylated
Hybridoma Cells Available: No
Antigen Species: Rat
Depositor: Hwang, Yu-wen
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: City University of New York
Positive Tested Species Reactivity: Human, Mouse, Rat
Depositors Notes: Liu Y, Adayev T and Hwang YW. An ELISA DYRK1A non-radioactive assay suitable for the characterization of inhibitors [version 1; referees: 2 approved]. F1000Research 2017, 6:42 (doi: 10.12688/f1000research.10582.1
Antigen Molecular Weight: 97.2 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Dnm1
Immunogen: Phosphorylated peptide
Alternate Gene Names: Dnm
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP/0
Epitope Location or Sequence: dynamin 1 amino acids 849-861
Uniprot ID: P21575 
Immunogen Sequence: RASPSRPES857PRPPC
Entrez Gene ID: 140694 
Additional Characterization: https://f1000research.com/articles/6-42/v1 
Antibody Registry ID: AB_2631263 
Additional Information: Dynamin 1 is phosphorylated by DYRK1A, a serine/threonine kinase.
Recommended Applications: ELISA, Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3D3 was deposited to the DSHB by Hwang, Yu-wen (DSHB Hybridoma Product 3D3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication
    IF References

    Activity-dependent phosphorylation of dynamin 1 at serine 857.
    Hwang YW
    Biochemistry 51.34 (2012 Aug 28): 6786-96.

    WB References
    All References

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