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SKU: 3D3-s
View product citations for antibody 3D3 on CiteAb

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Available: 28

DSHB Data Sheet

Catalog Fields

Antigen: dynamin-1xa, phosphorylated
Hybridoma Cells Available: No
Antigen Species: Rat
Depositor: Hwang, Yu-wen
Isotype: MIgG1
Depositors Institution: City University of New York
Host Species: mouse
Depositors Notes: Liu Y, Adayev T and Hwang YW. An ELISA DYRK1A non-radioactive assay suitable for the characterization of inhibitors [version 1; referees: 2 approved]. F1000Research 2017, 6:42 (doi: 10.12688/f1000research.10582.1
Positive Tested Species Reactivity: Human, Mouse, Rat
Gene: Dnm1
Antigen Molecular Weight: 97.2 kDa
Alternate Gene Names: Dnm
Immunogen: Phosphorylated peptide
Clonality: Monoclonal
Myeloma Strain: SP/0
Epitope Mapped: Yes
Uniprot ID: P21575 
Epitope Location or Sequence: dynamin 1 amino acids 849-861
Entrez Gene ID: 140694 
Immunogen Sequence: RASPSRPES857PRPPC
Antibody Registry ID: AB_2631263 
Additional Characterization: https://f1000research.com/articles/6-42/v1 
Recommended Applications: ELISA, Immunofluorescence, Western Blot
Additional Information: Dynamin 1 is phosphorylated by DYRK1A, a serine/threonine kinase.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3D3 was deposited to the DSHB by Hwang, Yu-wen (DSHB Hybridoma Product 3D3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication
    IF References

    Activity-dependent phosphorylation of dynamin 1 at serine 857.
    Hwang YW
    Biochemistry 51.34 (2012 Aug 28): 6786-96.

    WB References
    All References

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