Catalog Fields
Antigen: Lola zf5
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Johansen, Kristen
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Iowa State University
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This antigen is also known as longitudinals lacking protein (lola).
Predicted Species Reactivity:
Gene: Lola
Immunogen: Recombinant peptide
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: Sp2
Epitope Location or Sequence:
Uniprot ID: Q9V5M6
Immunogen Sequence: a.a. 427-748
Entrez Gene ID: 44548
Antibody Registry ID: AB_2721954
Additional Information: FBgn0283521 RRID:AB_2721954
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
7F1-1D5 was deposited to the DSHB by Johansen, Kristen (DSHB Hybridoma Product 7F1-1D5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
2 References
Initial Publication
A developmentally regulated splice variant from the complex lola locus encoding multiple different zinc finger domain proteins interacts with the chromosomal kinase JIL-1.
Johansen KM
The Journal of biological chemistry 278.13 (2003 Mar 28): 11696-704.