7F1-1D5

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SKU: 7F1-1D5

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Available: 35

DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: 7F1-1D5
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: Lola
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_2721954 
Uniprot ID: Q9V5M6 
RRID:  
Entrez Gene ID: 44548 
Clonality: Monoclonal
Immunogen: Recombinant peptide
Clone:
Immunogen Sequence: a.a. 427-748
Myeloma Strain: Sp2
Epitope Mapped: No
Antigen Name: Lola zf5
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 12/11/2015
Antigen Molecular Weight: apparent 105 kDa
Depositor: Johansen, Kristen
Antigen Sequence:
Depositor Institution: Iowa State University
Antigen Species: Drosophila
Depositor Notes: This antigen is also known as longitudinals lacking protein (lola).
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: FBgn0283521 RRID:AB_2721954
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
7F1-1D5 was deposited to the DSHB by Johansen, Kristen (DSHB Hybridoma Product 7F1-1D5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

2 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    IP References
    All References

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