7F1-1D5
Catalog Fields
Antigen: Lola zf5
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Johansen, Kristen
Isotype: MIgG1
Depositors Institution: Iowa State University
Host Species: mouse
Depositors Notes: This antigen is also known as longitudinals lacking protein (lola).
Positive Tested Species Reactivity: Drosophila
Gene: Lola
Antigen Molecular Weight: apparent 105 kDa
Clonality: Monoclonal
Immunogen: Recombinant peptide
Immunogen Sequence: a.a. 427-748
Myeloma Strain: Sp2
Additional Information: FBgn0283521 RRID:AB_2721954
Uniprot ID: Q9V5M6
Entrez Gene ID: 44548
Antibody Registry ID: AB_2721954
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
7F1-1D5 was deposited to the DSHB by Johansen, Kristen (DSHB Hybridoma Product 7F1-1D5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
2 References
Initial Publication
A developmentally regulated splice variant from the complex lola locus encoding multiple different zinc finger domain proteins interacts with the chromosomal kinase JIL-1.
Johansen KM
The Journal of biological chemistry 278.13 (2003 Mar 28): 11696-704.