9.2.1
Catalog Fields
Antigen: Myosin-2; myosin heavy chain C
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Miller, David, M., III
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Vanderbilt University
Positive Tested Species Reactivity: C. elegans
Depositors Notes: This antigen is also known as myosin heavy chain C (MHC C). The expression of this antigen is restricted to the pharynx.
Predicted Species Reactivity:
Gene: myo-2
Immunogen: Purified nematode myosin
Alternate Gene Names: CELE_T18D3.4
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: S194/5.XXO.Bu.1
Epitope Location or Sequence: LMM
Uniprot ID: P12845
Immunogen Sequence:
Entrez Gene ID: 181404
Additional Information: WormBase:WBGene00003514. Embryos permeabilized by freeze-crack, then post-fixed in ice-cold methanol followed by ice-cold acetone for 5 min. Embryos rehydrated by serial acetone dilution to water.
Recommended Applications: ELISA, Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
9.2.1 was deposited to the DSHB by Miller, David, M., III (DSHB Hybridoma Product 9.2.1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.
8 References
Initial Publication
Immunological identification of the genes encoding the four myosin heavy chain isoforms of Caenorhabditis elegans.
Karn J
Proceedings of the National Academy of Sciences of the United States of America 83.8 (1986 Apr): 2305-9.