9.2.1

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$40.00
SKU: 9.2.1
View product citations for antibody 9.2.1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Myosin-2; myosin heavy chain C
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Miller, David, M., III
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Vanderbilt University
Positive Tested Species Reactivity: C. elegans
Depositors Notes: This antigen is also known as myosin heavy chain C (MHC C). The expression of this antigen is restricted to the pharynx.
Antigen Molecular Weight: 210 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: myo-2
Immunogen: Purified nematode myosin
Alternate Gene Names: CELE_T18D3.4
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: S194/5.XXO.Bu.1
Epitope Location or Sequence: LMM
Uniprot ID: P12845 
Immunogen Sequence:
Entrez Gene ID: 181404 
Additional Characterization:
Antibody Registry ID:
Additional Information: WormBase:WBGene00003514. Embryos permeabilized by freeze-crack, then post-fixed in ice-cold methanol followed by ice-cold acetone for 5 min. Embryos rehydrated by serial acetone dilution to water.
Recommended Applications: ELISA, Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
9.2.1 was deposited to the DSHB by Miller, David, M., III (DSHB Hybridoma Product 9.2.1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

8 References

  • Initial Publication
  • IF References
  • WB References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication

    Proceedings of the National Academy of Sciences of the United States of America
    Karn J
    Proceedings of the National Academy of Sciences of the United States of America 83.8 (1986 Apr): 2305-9.

    IF References

    Developmental biology
    Mango SE
    Developmental biology 303.2 (2007 Mar 15): 611-24.

    Proceedings of the National Academy of Sciences of the United States of America
    Karn J
    Proceedings of the National Academy of Sciences of the United States of America 83.8 (1986 Apr): 2305-9.

    Developmental biology
    Okkema PG
    Developmental biology 276.2 (2004 Dec 15): 352-66.

    The Journal of cell biology
    Epstein HF
    The Journal of cell biology 105.6 Pt 1 (1987 Dec): 2763-70.

    Development (Cambridge, England)
    Austin J
    Development (Cambridge, England) 125.11 (1998 Jun): 2087-98.

    Proceedings of the National Academy of Sciences of the United States of America
    Okkema PG
    Proceedings of the National Academy of Sciences of the United States of America 95.9 (1998 Apr 28): 5072-5.

    Development (Cambridge, England)
    McGhee JD
    Development (Cambridge, England) 125.12 (1998 Jun): 2171-80.

    WB References

    Proceedings of the National Academy of Sciences of the United States of America
    Karn J
    Proceedings of the National Academy of Sciences of the United States of America 83.8 (1986 Apr): 2305-9.

    ELISA References

    Cell
    Epstein HF
    Cell 34.2 (1983 Sep): 477-90.

    Epitope Map References

    Proceedings of the National Academy of Sciences of the United States of America
    Karn J
    Proceedings of the National Academy of Sciences of the United States of America 83.8 (1986 Apr): 2305-9.

    All References

    Developmental biology
    Mango SE
    Developmental biology 303.2 (2007 Mar 15): 611-24.

    Proceedings of the National Academy of Sciences of the United States of America
    Karn J
    Proceedings of the National Academy of Sciences of the United States of America 83.8 (1986 Apr): 2305-9.

    Developmental biology
    Okkema PG
    Developmental biology 276.2 (2004 Dec 15): 352-66.

    The Journal of cell biology
    Epstein HF
    The Journal of cell biology 105.6 Pt 1 (1987 Dec): 2763-70.

    Development (Cambridge, England)
    Austin J
    Development (Cambridge, England) 125.11 (1998 Jun): 2087-98.

    Proceedings of the National Academy of Sciences of the United States of America
    Okkema PG
    Proceedings of the National Academy of Sciences of the United States of America 95.9 (1998 Apr 28): 5072-5.

    Development (Cambridge, England)
    McGhee JD
    Development (Cambridge, England) 125.12 (1998 Jun): 2171-80.

    Cell
    Epstein HF
    Cell 34.2 (1983 Sep): 477-90.

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