9.2.1
Catalog Fields
Antigen: Myosin-2; myosin heavy chain C
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Miller, David, M., III
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Vanderbilt University
Positive Tested Species Reactivity: C. elegans
Depositors Notes: This antigen is also known as myosin heavy chain C (MHC C). The expression of this antigen is restricted to the pharynx.
Antigen Molecular Weight: 210 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Gene: myo-2
Immunogen: Purified nematode myosin
Alternate Gene Names: CELE_T18D3.4
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: S194/5.XXO.Bu.1
Epitope Location or Sequence: LMM
Uniprot ID: P12845
Immunogen Sequence:
Entrez Gene ID: 181404
Additional Characterization:
Antibody Registry ID:
Additional Information: WormBase:WBGene00003514. Embryos permeabilized by freeze-crack, then post-fixed in ice-cold methanol followed by ice-cold acetone for 5 min. Embryos rehydrated by serial acetone dilution to water.
Recommended Applications: ELISA, Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
9.2.1 was deposited to the DSHB by Miller, David, M., III (DSHB Hybridoma Product 9.2.1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
8 References
Initial Publication
Immunological identification of the genes encoding the four myosin heavy chain isoforms of Caenorhabditis elegans.
Karn J
Proceedings of the National Academy of Sciences of the United States of America 83.8 (1986 Apr): 2305-9.