9E9-1B11

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$40.00
SKU: 9E9-1B11
View product citations for antibody 9E9-1B11 on CiteAb

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Available: 41

DSHB Data Sheet

Catalog Fields

Antigen: D-Hillarin
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Johansen, Kristen
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Iowa State University
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This antigen is localized to the CNS in Drosophila and to the cleavage furrow in dividing S2 cells.
Antigen Molecular Weight: apparent 97 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Hil
Immunogen: Recombinant peptide
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: Sp2
Epitope Location or Sequence:
Uniprot ID: Q0E908 
Immunogen Sequence: a.a. 222-426
Entrez Gene ID: 37328 
Additional Characterization:  
Antibody Registry ID: AB_2721956 
Additional Information: FBgn0050147 RRID:AB_2721956
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
9E9-1B11 was deposited to the DSHB by Johansen, Kristen (DSHB Hybridoma Product 9E9-1B11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • IF References
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