9S4
Catalog Fields
Antigen: Selenoprotein P
Hybridoma Cells Available: Yes
Antigen Species: mouse
Depositor: Burke, R.D.
Isotype: RIgG1, kappa light chain
Antigen Sequence:
Host Species: rat
Depositors Institution: Vanderbilt University
Positive Tested Species Reactivity: Mouse
Depositors Notes: Protocol in G.E. Olson, et al. JBC 282:12290-12297 (2007)
Antigen Molecular Weight: 42.7
Human Protein Atlas:
Predicted Species Reactivity:
Gene: SEPP1
Immunogen: Polypeptide
Alternate Gene Names: SELP, Se-P
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63-Ag8U.1
Epitope Location or Sequence: N-terminal region
Uniprot ID: P70274
Immunogen Sequence:
Entrez Gene ID: 20363
Additional Characterization:
Antibody Registry ID: AB_2617215
Additional Information: RRID:AB_2617215
Recommended Applications: ELISA, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
9S4 was deposited to the DSHB by Burke, R.D. (DSHB Hybridoma Product 9S4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
3 References
Initial Publication
The selenium-rich C-terminal domain of mouse selenoprotein P is necessary for the supply of selenium to brain and testis but not for the maintenance of whole body selenium.
Burk RF
The Journal of biological chemistry 282.15 (2007 Apr 13): 10972-80.