9S4

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$40.00
SKU: 9S4
View product citations for antibody 9S4 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Selenoprotein P
Hybridoma Cells Available: Yes
Antigen Species: mouse
Depositor: Burke, R.D.
Isotype: RIgG1, kappa light chain
Antigen Sequence:
Host Species: rat
Depositors Institution: Vanderbilt University
Positive Tested Species Reactivity: Mouse
Depositors Notes: Protocol in G.E. Olson, et al. JBC 282:12290-12297 (2007)
Antigen Molecular Weight: 42.7
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: SEPP1
Immunogen: Polypeptide
Alternate Gene Names: SELP, Se-P
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63-Ag8U.1
Epitope Location or Sequence: N-terminal region
Uniprot ID: P70274 
Immunogen Sequence:
Entrez Gene ID: 20363 
Additional Characterization:  
Antibody Registry ID: AB_2617215 
Additional Information: RRID:AB_2617215
Recommended Applications: ELISA, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
9S4 was deposited to the DSHB by Burke, R.D. (DSHB Hybridoma Product 9S4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • ELISA References
  • All References
  • Initial Publication
    IF References

    Maternal-fetal transfer of selenium in the mouse.
    Kurokawa S
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 27.8 (2013 Aug): 3249-56.

    WB References
    IP References
    ELISA References
    All References

    Maternal-fetal transfer of selenium in the mouse.
    Kurokawa S
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 27.8 (2013 Aug): 3249-56.

    The selenium-rich C-terminal domain of mouse selenoprotein P is necessary for the supply of selenium to brain and testis but not for the maintenance of whole body selenium.
    Burk RF
    The Journal of biological chemistry 282.15 (2007 Apr 13): 10972-80.

    Production of selenoprotein P (Sepp1) by hepatocytes is central to selenium homeostasis.
    Burk RF
    The Journal of biological chemistry 287.48 (2012 Nov 23): 40414-24.

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