A4.1519

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$40.00
SKU: A4.1519
View product citations for antibody A4.1519 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Myosin heavy chain (human nascent secondary and all fast fibers)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Blau, H.M.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: Baxter Lab for Stem Cell Biology, Stanford University
Positive Tested Species Reactivity: Human, Porcine, Rat, Rodent
Depositors Notes: Fusion: 1983. Recognizes nascent secondary and all fast adult fibers in human. In rat, high affinity for IIA fibers, low affinity for IIB fibers, and doesn't stain IIX/D.
Antigen Molecular Weight: 220kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: MYH2
Immunogen: partially purified myosin (pyrophosphate extracted) from adult (22 year old) skeletal muscle
Alternate Gene Names: BM3; MYH2A; MYPOP; MYHSA2; MYHas8; MyHC-2A; MyHC-IIa
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: 653
Epitope Location or Sequence: LMM region a.a. 1661-1758
Uniprot ID: Q9UKX2 
Immunogen Sequence:
Entrez Gene ID: 4620 
Additional Characterization:
Antibody Registry ID: AB_528357 
Additional Information: This antibody may recognize more than one myosin isoform depending on the tissue, developmental stage, or species. The gene, Uniprot ID and gene ID associated with this mAb are not exclusive.
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
A4.1519 was deposited to the DSHB by Blau, H.M. (DSHB Hybridoma Product A4.1519)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

9 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References

    Postnatal development of myosin heavy chain isoforms in rat laryngeal muscles.
    Flint PW
    The Annals of otology, rhinology, and laryngology 108.5 (1999 May): 509-15.

    Fast muscle fibers are preferentially affected in Duchenne muscular dystrophy.
    Blau HM
    Cell 52.4 (1988 Feb 26): 503-13.

    Chronic congestive heart failure elicits adaptations of endurance exercise in diaphragmatic muscle.
    Mancini D
    Circulation 95.4 (1997 Feb 18): 910-6.

    Myosin heavy chain composition in human laryngeal muscles.
    Flint PW
    The Laryngoscope 109.9 (1999 Sep): 1521-4.

    Changes in myofibrillar protein composition of human diaphragm elicited by congestive heart failure.
    Levine S
    Journal of molecular and cellular cardiology 28.12 (1996 Dec): 2537-41.

    Method for decellularizing skeletal muscle without detergents or proteolytic enzymes.
    Varghese S
    Tissue engineering. Part C, Methods 17.4 (2011 Apr): 383-9.

    Fast myosin heavy chains expressed in secondary mammalian muscle fibers at the time of their inception.
    Blau HM
    Journal of cell science 107 ( Pt 9). (1994 Sep): 2361-71.

    IHC References
    ELISA References
    Epitope Map References
    All References

    Muscle fiber pattern is independent of cell lineage in postnatal rodent development.
    Blau HM
    Cell 68.4 (1992 Feb 21): 659-71.

    Significant differences among skeletal muscles in the incorporation of bone marrow-derived cells.
    Blau HM
    Developmental biology 262.1 (2003 Oct 1): 64-74.

    Fast myosin heavy chains expressed in secondary mammalian muscle fibers at the time of their inception.
    Blau HM
    Journal of cell science 107 ( Pt 9). (1994 Sep): 2361-71.

    Postnatal development of myosin heavy chain isoforms in rat laryngeal muscles.
    Flint PW
    The Annals of otology, rhinology, and laryngology 108.5 (1999 May): 509-15.

    Fast muscle fibers are preferentially affected in Duchenne muscular dystrophy.
    Blau HM
    Cell 52.4 (1988 Feb 26): 503-13.

    Chronic congestive heart failure elicits adaptations of endurance exercise in diaphragmatic muscle.
    Mancini D
    Circulation 95.4 (1997 Feb 18): 910-6.

    Myosin heavy chain composition in human laryngeal muscles.
    Flint PW
    The Laryngoscope 109.9 (1999 Sep): 1521-4.

    Changes in myofibrillar protein composition of human diaphragm elicited by congestive heart failure.
    Levine S
    Journal of molecular and cellular cardiology 28.12 (1996 Dec): 2537-41.

    Method for decellularizing skeletal muscle without detergents or proteolytic enzymes.
    Varghese S
    Tissue engineering. Part C, Methods 17.4 (2011 Apr): 383-9.

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