A4.1519

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SKU: A4.1519

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DSHB Data Sheet

Catalog Fields

Product Name/ID: A4.1519
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: MYH2
Ab Isotype: MIgM
Gene Symbol:
Antibody Registry ID: AB_528357 
Uniprot ID: Q9UKX2 
RRID:  
Entrez Gene ID: 4620 
Clonality: Monoclonal
Immunogen: partially purified myosin (pyrophosphate extracted) from adult (22 year old) skeletal muscle
Clone:
Immunogen Sequence:
Myeloma Strain: 653
Epitope Mapped: Yes
Antigen Name: Myosin heavy chain (human nascent secondary and all fast fibers)
Epitope Location or Sequence: LMM region a.a. 1661-1758
Alternate Antigen Name:
Deposit Date: 8/25/1993
Antigen Molecular Weight: 220kDa
Depositor: Blau, H.M.
Antigen Sequence:
Depositor Institution: Stanford University, Baxter Lab for Stem Cell Biology
Antigen Species: Human
Depositor Notes: Fusion: 1983. Recognizes nascent secondary and all fast adult fibers in human. In rat, high affinity for IIA fibers, low affinity for IIB fibers, and doesn't stain IIX/D.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Porcine, Rat, Rodent
Additional Information: This antibody may recognize more than one myosin isoform depending on the tissue, developmental stage, or species. The gene, Uniprot ID and gene ID associated with this mAb are not exclusive.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
A4.1519 was deposited to the DSHB by Blau, H.M. (DSHB Hybridoma Product A4.1519)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

9 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References

    Postnatal development of myosin heavy chain isoforms in rat laryngeal muscles.
    Flint PW
    The Annals of otology, rhinology, and laryngology 108.5 (1999 May): 509-15.

    Fast muscle fibers are preferentially affected in Duchenne muscular dystrophy.
    Blau HM
    Cell 52.4 (1988 Feb 26): 503-13.

    Chronic congestive heart failure elicits adaptations of endurance exercise in diaphragmatic muscle.
    Mancini D
    Circulation 95.4 (1997 Feb 18): 910-6.

    Myosin heavy chain composition in human laryngeal muscles.
    Flint PW
    The Laryngoscope 109.9 (1999 Sep): 1521-4.

    Changes in myofibrillar protein composition of human diaphragm elicited by congestive heart failure.
    Levine S
    Journal of molecular and cellular cardiology 28.12 (1996 Dec): 2537-41.

    Method for decellularizing skeletal muscle without detergents or proteolytic enzymes.
    Varghese S
    Tissue engineering. Part C, Methods 17.4 (2011 Apr): 383-9.

    Fast myosin heavy chains expressed in secondary mammalian muscle fibers at the time of their inception.
    Blau HM
    Journal of cell science 107 ( Pt 9). (1994 Sep): 2361-71.

    IHC References
    ELISA References
    Epitope Map References
    All References

    Muscle fiber pattern is independent of cell lineage in postnatal rodent development.
    Blau HM
    Cell 68.4 (1992 Feb 21): 659-71.

    Significant differences among skeletal muscles in the incorporation of bone marrow-derived cells.
    Blau HM
    Developmental biology 262.1 (2003 Oct 1): 64-74.

    Fast myosin heavy chains expressed in secondary mammalian muscle fibers at the time of their inception.
    Blau HM
    Journal of cell science 107 ( Pt 9). (1994 Sep): 2361-71.

    Postnatal development of myosin heavy chain isoforms in rat laryngeal muscles.
    Flint PW
    The Annals of otology, rhinology, and laryngology 108.5 (1999 May): 509-15.

    Fast muscle fibers are preferentially affected in Duchenne muscular dystrophy.
    Blau HM
    Cell 52.4 (1988 Feb 26): 503-13.

    Chronic congestive heart failure elicits adaptations of endurance exercise in diaphragmatic muscle.
    Mancini D
    Circulation 95.4 (1997 Feb 18): 910-6.

    Myosin heavy chain composition in human laryngeal muscles.
    Flint PW
    The Laryngoscope 109.9 (1999 Sep): 1521-4.

    Changes in myofibrillar protein composition of human diaphragm elicited by congestive heart failure.
    Levine S
    Journal of molecular and cellular cardiology 28.12 (1996 Dec): 2537-41.

    Method for decellularizing skeletal muscle without detergents or proteolytic enzymes.
    Varghese S
    Tissue engineering. Part C, Methods 17.4 (2011 Apr): 383-9.

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