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SKU: AFFN-AKT3-18F11-s
View product citations for antibody AFFN-AKT3-18F11 on CiteAb

In Stock

Available: 39

DSHB Data Sheet

Catalog Fields

Antigen: RAC-gamma serine/threonine-protein kinase
Hybridoma Cells Available:
Antigen Species: Human
Depositor: EU Program Affinomics
Isotype: MIgG2c
Antigen Sequence:
Host Species: mouse
Depositors Institution: EMBL MACF
Positive Tested Species Reactivity: Human
Depositors Notes: This antigen is also known as v-akt murine thymoma viral oncogene homolog 3 or PKB gamma.
Antigen Molecular Weight: 55.7 kDa
Human Protein Atlas:
Predicted Species Reactivity: Mouse, Porcine, Rabbit, Zebrafish
Gene: AKT3
Immunogen: E. coli expressed recombinant protein
Alternate Gene Names: MPPH, MPPH2, PKB-GAMMA, PKBG, PRKBG, RAC-gamma, RAC-PK-gamma, STK-2
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: SP2/0-AG14
Epitope Location or Sequence:
Uniprot ID: Q9Y243 
Immunogen Sequence: 40 kDa Protein fragment
Entrez Gene ID: 10000 
Additional Characterization:
Antibody Registry ID: AB_2617438 
Additional Information: Akt3 is a member of the RAS pathway related proteins. RRID:AB_2617438
Recommended Applications: ELISA, Microarray
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
AFFN-AKT3-18F11 was deposited to the DSHB by EU Program Affinomics (DSHB Hybridoma Product AFFN-AKT3-18F11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • All References
  • Initial Publication

    Nature methods
    Gräslund S
    Nature methods 8.7 (2011 May 15): 551-8.

    Nature methods
    Uhlén M
    Nature methods 4.1 (2007 Jan): 13-7.

    All References

    Nature methods
    Gräslund S
    Nature methods 8.7 (2011 May 15): 551-8.

    Nature methods
    Uhlén M
    Nature methods 4.1 (2007 Jan): 13-7.

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