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View product citations for antibody AFFN-DLG1-4D6 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Discs, large homolog 1
Hybridoma Cells Available: No
Antigen Species: Human
Depositor: EU Program Affinomics
Isotype: MIgG2c
Depositors Institution: EMBL MACF
Host Species: mouse
Gene: DLG1
Positive Tested Species Reactivity: Human
Alternate Gene Names: dJ1061C18.1.1, DLGH1, hdlg, SAP-97, SAP97
Antigen Molecular Weight: Predicted: 100.5kDa
Clonality: Monoclonal
Predicted Species Reactivity: Porcine, Rabbit
Epitope Mapped: No
Immunogen: E. coli expressed recombinant protein
Immunogen Sequence: a.a. 224-312
Myeloma Strain: SP2/0-AG14
Uniprot ID: Q12959 
Additional Information: This antigen is the human homolog of the Drosophila protein discs large 1. RRID:AB_2617529
Entrez Gene ID: 1739 
Antibody Registry ID: AB_2617529 
Recommended Applications: ELISA, Microarray
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
AFFN-DLG1-4D6 was deposited to the DSHB by EU Program Affinomics (DSHB Hybridoma Product AFFN-DLG1-4D6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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