AFFN-PARP1-17B10

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$40.00
SKU: AFFN-PARP1-17B10-s
View product citations for antibody AFFN-PARP1-17B10 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Poly (ADP-ribose) polymerase 1
Hybridoma Cells Available:
Antigen Species: Human
Depositor: EU Program Affinomics
Isotype: MIgG
Antigen Sequence:
Host Species: mouse
Depositors Institution: EMBL MACF
Positive Tested Species Reactivity: Human
Depositors Notes: This antibody recognizes a polymerase involved in base excision repair.
Antigen Molecular Weight: 113.0 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: PARP1
Immunogen: Recombinant peptide
Alternate Gene Names: ADPRT, PARP, PPOL
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: SP2/0-AG14
Epitope Location or Sequence:
Uniprot ID: P09874 
Immunogen Sequence: a.a. 384-495
Entrez Gene ID: 142 
Additional Characterization: Link 
Antibody Registry ID: AB_2617801 
Additional Information: RRID:AB_2617801
Recommended Applications: ELISA, Microarray
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
AFFN-PARP1-17B10 was deposited to the DSHB by EU Program Affinomics (DSHB Hybridoma Product AFFN-PARP1-17B10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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