AFFN-PHF8-9H6

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$40.00
SKU: AFFN-PHF8-9H6-s
View product citations for antibody AFFN-PHF8-9H6 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Histone lysine demethylase PHF8
Hybridoma Cells Available:
Antigen Species: Human
Depositor: EU Program Affinomics
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: EMBL MACF
Positive Tested Species Reactivity: Human
Depositors Notes: This antibody recognizes a histone lysine demethylase that acts on histone in the monomethyl or dimethyl states.
Antigen Molecular Weight: 117.8 kDa
Human Protein Atlas:
Predicted Species Reactivity: Porcine, Rabbit
Gene: PHF8
Immunogen: Recombinant peptide
Alternate Gene Names: JHDM1F, ZNF422
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: SP2/0-AG14
Epitope Location or Sequence:
Uniprot ID: Q9UPP1 
Immunogen Sequence: a.a. 750-858
Entrez Gene ID: 23133 
Additional Characterization: Link 
Antibody Registry ID: AB_2617817 
Additional Information: RRID:AB_2617817
Recommended Applications: ELISA, Microarray
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
AFFN-PHF8-9H6 was deposited to the DSHB by EU Program Affinomics (DSHB Hybridoma Product AFFN-PHF8-9H6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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