Antigen: PR domain zinc finger protein 8
Available to For-Profits: Yes
Antigen Species: Human
Hybridoma Cells Available (Non-Profit): No
Depositor: EU Program Affinomics
Host Species: mouse
Positive Tested Species Reactivity: Human
Depositors Institution: EMBL MACF
Antigen Molecular Weight: 71.66 kDa
Depositors Notes: This antigen is also known as PR domain containing 8.
Immunogen: E. coli expressed recombinant protein
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Myeloma Strain: SP2/0-AG14
Epitope Mapped: No
Uniprot ID: Q9NQV8
Epitope Location or Sequence:
Entrez Gene ID: 56978
Immunogen Sequence: Full length
Antibody Registry ID: AB_2617851
Additional Characterization: https://cordis.europa.eu/project/id/241481/reporting
Recommended Applications: ELISA, Microarray
Additional Information: RRID:AB_2617851
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
AFFN-PRDM8-10F5 was deposited to the DSHB by EU Program Affinomics (DSHB Hybridoma Product AFFN-PRDM8-10F5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).