AFFN-SHC4-9E1

(0)No Reviews yet
$45.00
SKU: AFFN-SHC4-9E1-s
View product citations for antibody AFFN-SHC4-9E1 on CiteAb

Please allow 4 -6 weeks for production and delivery.

DSHB Data Sheet

Catalog Fields

Product Name/ID: AFFN-SHC4-9E1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: SHC4
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_2617894 
Uniprot ID: Q6S5L8 
RRID:  
Entrez Gene ID: 399694 
Clonality: Monoclonal
Immunogen: E. coli expressed recombinant protein
Clone:
Immunogen Sequence: a.a. 512-630; SH2 domain
Myeloma Strain: SP2/0-AG14
Epitope Mapped: No
Antigen Name: SHC (Src homology 2 domain containing) family, member 4
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 11/13/2014
Antigen Molecular Weight: Predicted: 68.7kDa
Depositor: EU Program Affinomics
Antigen Sequence:
Depositor Institution: EMBL MACF
Antigen Species: Human
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Human
Additional Information: RRID:AB_2617894
Predicted Species Reactivity: Rabbit
Human Protein Atlas:  
Recommended Applications: ELISA, Microarray
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
AFFN-SHC4-9E1 was deposited to the DSHB by EU Program Affinomics (DSHB Hybridoma Product AFFN-SHC4-9E1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

2 References

  • Initial Publication
  • All References
  • Ratings & Reviews

    No reviews available

    Be the first to Write a Review