ANTI-GRL(2)
Catalog Fields
Antigen: leukocyte, activated, cell surface glycoprotein
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Thomas, J.-L.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: INSERM U495, Hopital de la Salpetriere
Positive Tested Species Reactivity: Chicken
Depositors Notes: Cell binding note: thrombocytes, granulocytes, activated T cells, exocrine, endocrine cells. Antigen chemical properties: highly N-glycosylated protein.
Predicted Species Reactivity:
Gene:
Immunogen: bursa of Fabricius
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Antibody Registry ID: AB_2110620
Additional Information:
Recommended Applications: Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ANTI-GRL(2) was deposited to the DSHB by Thomas, J.-L. (DSHB Hybridoma Product ANTI-GRL(2))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.
1 Reference
Initial Publication
Identification in the chicken of GRL1 and GRL2: two granule proteins expressed on the surface of activated leukocytes.
Le Douarin NM
Experimental cell research 204.1 (1993 Jan): 156-66.