ASCS4

(0) No Reviews yet
$45.00 to $425.00
SKU: ASCS4
View product citations for antibody ASCS4 on CiteAb

In Stock

Available: 24

DSHB Data Sheet

Catalog Fields

Antigen: L1 protein (rat)
Available to For-Profits: Yes
Antigen Species: rat
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Patterson, P.H.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Rat
Depositors Institution: California Institute of Technology
Antigen Molecular Weight: 200 kDa, 130 kDa, 85 kDa
Depositors Notes:
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: sympathetic neurons, rat SCG (superior cervical ganglion)
Gene:
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: NS1
Epitope Mapped: No
Uniprot ID:  
Epitope Location or Sequence:
Entrez Gene ID:  
Immunogen Sequence:
Antibody Registry ID: AB_528349 
Additional Characterization:  
Recommended Applications: Immunohistochemistry
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
ASCS4 was deposited to the DSHB by Patterson, P.H. (DSHB Hybridoma Product ASCS4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    Post-translational modification and evoked release of two large surface proteins of sympathetic neurons.
    Sweadner KJ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 3.12 (1983 Dec): 2504-17.

    All References

    Post-translational modification and evoked release of two large surface proteins of sympathetic neurons.
    Sweadner KJ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 3.12 (1983 Dec): 2504-17.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review