Anti-GAD1 [BCN49.2.3D1]

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SKU: Anti-GAD1 [BCN49.2.3D1]

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DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: Anti-GAD1 [BCN49.2.3D1]
Available to For-Profits: Yes
Alternate Antibody Name: BCN49.2.3D1
Gene Symbol: GAD1
Ab Isotype: MIgG2c
Gene Name:
Antibody Registry ID: AB_3099720 
Uniprot ID: Q99259 
Entrez Gene ID: 2571 
Clonality: Monoclonal
Immunogen: Recombinant protein produced in yeast
Immunogen Sequence:
Myeloma Strain: SP2/0-Ag14
Epitope Mapped: No
Antigen Name: Glutamate decarboxylase 1
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 2/28/2022
Antigen Molecular Weight: 66.8 kDa
Depositor: Pino, I.
Antigen Sequence:
Depositor Institution: CDI Labs
Antigen Species: Human
Depositor Notes: Catalyzes the synthesis of the inhibitor transmitter GABA.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information: Mouse strain CD1 was used as the host.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Anti-GAD1 [BCN49.2.3D1] was deposited to the DSHB by Pino, I. (DSHB Hybridoma Product Anti-GAD1 [BCN49.2.3D1])
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.

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