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SKU: B6E11

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DSHB Data Sheet

Catalog Fields

Product Name/ID: B6E11
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: SLC10A1
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_2617432 
Uniprot ID: Q14973 
Entrez Gene ID: 6554 
Clonality: Monoclonal
Immunogen: Peptide
Immunogen Sequence: YSRGIYDGDLKDKVPY
Myeloma Strain: unknown
Epitope Mapped: No
Antigen Name: Sodium/bile acid cotransporter
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 8/9/2015
Antigen Molecular Weight: 38.1 kDa
Depositor: Duncan, S.
Antigen Sequence:
Depositor Institution: Medical University of South Carolina
Antigen Species: Human
Depositor Notes: Antibodies have been tested by immunocytochemistry and flow cytometry.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information: Hybridomas were generated by Biomatik under contract from the Duncan Lab. RRID:AB_2617432
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: FACS, Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
B6E11 was deposited to the DSHB by Duncan, S. (DSHB Hybridoma Product B6E11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

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