Antigen: Actin, indirect flight muscle; Actin-88F
Hybridoma Cells Available: Yes
Antigen Species: Lethocerus indicus
Depositor: Bullard, Belinda
Host Species: rat
Depositors Institution: University of York
Positive Tested Species Reactivity: Drosophila, Lethocerus indicus
Depositors Notes: This antibody is also known as MAC 237. This antibody is validated in the citation j Exp Biol 168, 57-75
Predicted Species Reactivity:
Alternate Gene Names: actin
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: Y3Agl.2.3.
Epitope Location or Sequence:
Uniprot ID: P83967
Entrez Gene ID: 41885
Antibody Registry ID: AB_2889798
Additional Information: RRID: AB_2889798, FBgn0000047.
Recommended Applications: Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
BB8/20.11 was deposited to the DSHB by Bullard, Belinda (DSHB Hybridoma Product BB8/20.11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).