BCN21.2.1A11

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SKU: BCN21.2.1A11-s

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Available: 11

DSHB Data Sheet

Catalog Fields

Product Name/ID: BCN21.2.1A11
Available to For-Profits: No
Alternate Antibody Name:
Gene Name: RBFOX3
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_3083094 
Uniprot ID: A6NFN3 
RRID:  
Entrez Gene ID: 146713 
Clonality: Monoclonal
Immunogen: Recombinant protein produced in yeast
Clone:
Immunogen Sequence: Human RBFOX3 protein fragment (aa 3-110)
Myeloma Strain: SP2/0-Ag14
Epitope Mapped: No
Antigen Name: RNA binding protein fox-1 homolog 3/ NeuN
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 4/7/2022
Antigen Molecular Weight: 33.8 kDa
Depositor: Pino, I.
Antigen Sequence:
Depositor Institution: CDI Labs
Antigen Species: Human
Depositor Notes: Pre-mRNA alternative splicing regulator. Regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD).
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Mouse
Additional Information: Mouse strain CD1 was used.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
BCN21.2.1A11 was deposited to the DSHB by Pino, I. (DSHB Hybridoma Product BCN21.2.1A11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.

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