Broad-Z3 (Z3.9A7)

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SKU: Broad-Z3 (Z3.9A7)

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DSHB Data Sheet

Catalog Fields

Product Name/ID: Broad-Z3 (Z3.9A7)
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: br
Ab Isotype: MIgG2a, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_528106 
Uniprot ID: Q01295 
RRID:  
Entrez Gene ID: 44505 
Clonality: Monoclonal
Immunogen: Recombinant, His-tagged fusion protein derived from cDNA clone Z3 corresponding to aa 516-702 of isoform 5 of BR-C [Uniprot ID Q01295-5]
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain: Sp2/0-Ag14
Epitope Mapped: Yes
Antigen Name: Broad (Z3)
Epitope Location or Sequence: aa 516-702 of isoform 5 [Uniprot ID Q01295-5]
Alternate Antigen Name:
Deposit Date: 10/15/2003
Antigen Molecular Weight: Predicted: 74 kDa; Apparent: 91 kDa
Depositor: Guild, G.
Antigen Sequence:
Depositor Institution: University of Pennsylvania
Antigen Species: Drosophila
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Drosophila
Additional Information: Specific for the Z3 isoform of the broad protein complex of transcription factors. There is one Z3 BR-C isoform reported.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Broad-Z3 (Z3.9A7) was deposited to the DSHB by Guild, G. (DSHB Hybridoma Product Broad-Z3 (Z3.9A7))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

4 References

  • Initial Publication
  • IF References
  • WB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    Epitope Map References
    All References

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