Broad-Z3 (Z3.9A7)

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SKU: Broad-Z3 (Z3.9A7)
View product citations for antibody Broad-Z3 (Z3.9A7) on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Broad (Z3)
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Guild, G.
Isotype: MIgG2a, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Pennsylvania
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: Predicted: 74 kDa; Apparent: 91 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: br
Immunogen: Recombinant, His-tagged fusion protein derived from cDNA clone Z3 corresponding to aa 516-702 of isoform 5 of BR-C [Uniprot ID Q01295-5]
Alternate Gene Names: CG11509; BR-C; BR-C Z1; Br-C Z2; Br-C-Z3; npr1; PP1; PP2; rbp; rdp; rds; uq
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Sp2/0-Ag14
Epitope Location or Sequence: aa 516-702 of isoform 5 [Uniprot ID Q01295-5]
Uniprot ID: Q01295 
Immunogen Sequence: Partial protein
Entrez Gene ID: 44505 
Additional Characterization:  
Antibody Registry ID: AB_528106 
Additional Information: Specific for the Z3 isoform of the broad protein complex of transcription factors. There is one Z3 BR-C isoform reported.
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Broad-Z3 (Z3.9A7) was deposited to the DSHB by Guild, G. (DSHB Hybridoma Product Broad-Z3 (Z3.9A7))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • IF References
  • WB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    Epitope Map References
    All References

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