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View product citations for antibody C1B7 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Acetylcholinesterase
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: August, J.T.
Isotype: MIgG1, kappa light chain
Depositors Institution: Johns Hopkins University School of Medicine
Host Species: mouse
Depositors Notes: This antibody inhibits AChE activity up to 75%.
Positive Tested Species Reactivity: Human
Gene: ACHE
Antigen Molecular Weight: 67.7 kDa
Alternate Gene Names: YT
Immunogen: Erythrocyte membrane ghosts
Clonality: Monoclonal
Myeloma Strain: P3x63-Ag8-653
Epitope Mapped: No
Uniprot ID: P22303 
Additional Information: RRID:AB_528065
Entrez Gene ID: 43 
Antibody Registry ID: AB_528065 
Recommended Applications: Function Blocking, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
C1B7 was deposited to the DSHB by August, J.T. (DSHB Hybridoma Product C1B7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
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