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View product citations for antibody C3B6 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Sodium/bile acid cotransporter
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Duncan, S.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Medical University of South Carolina
Positive Tested Species Reactivity: Human
Depositors Notes: Antibodies have been tested by immunocytochemistry and flow cytometry. The epitope is predicted to recognize an extracellular epitope.
Antigen Molecular Weight: 38.1 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: SLC10A1
Immunogen: Peptide
Alternate Gene Names: NTCP, Growth-Inhibiting Protein 29, Na(+)/Taurocholate Transport Protein
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: unknown
Epitope Location or Sequence:
Uniprot ID: Q14973 
Immunogen Sequence: YSRGIYDGDLKDKVPY
Entrez Gene ID: 6554 
Additional Characterization:  
Antibody Registry ID: AB_2617433 
Additional Information: Hybridomas were generated by Biomatik under contract from the Duncan Lab. RRID:AB_2617433. SLC10A1 has been described as a receptor for Hepatitis B virus.
Recommended Applications: Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
C3B6 was deposited to the DSHB by Duncan, S. (DSHB Hybridoma Product C3B6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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