CH106

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$40.00
SKU: CH106
View product citations for antibody CH106 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: tropomyosin
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Lin, J.J.-C.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Iowa
Positive Tested Species Reactivity: Broad species, Chicken, Human, Mouse
Depositors Notes:
Antigen Molecular Weight: Apparent: 39 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: TPM1
Immunogen: Purified chicken cardiac muscle tropomyosin
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS1
Epitope Location or Sequence:
Uniprot ID: P04268 
Immunogen Sequence: Full length protein
Entrez Gene ID: 396366 
Additional Characterization:  
Antibody Registry ID: AB_10804668 
Additional Information: CH106 recognizes the fast-migrating component of gizzard tropomyosin and cardiac alpha-tropomyosin and skeletal beta-tropomyosin, but not skeletal alpha-tropomyosin or chicken embryo fibroblasts tropomyosin. CH106 interferes with the binding of tropomyosin to actin.
Recommended Applications: Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CH106 was deposited to the DSHB by Lin, J.J.-C. (DSHB Hybridoma Product CH106)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

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