Antigen: Brain-Specific Angiogenesis Inhibitor 1
Hybridoma Cells Available: No
Antigen Species: Human
Depositor: Clinical Proteomics Technologies for Cancer
Depositors Institution: National Cancer Institute
Host Species: mouse
Depositors Notes: This antigen is also known as adhesion G protein-coupled receptor B1 and is cleaved into vasculostatin-40.
Positive Tested Species Reactivity: Human
Antigen Molecular Weight: 173.5 kDa
Alternate Gene Names: BAI1
Immunogen: Synthetic Peptide
Myeloma Strain: P3x63Ag8.653
Epitope Mapped: Yes
Uniprot ID: O14514
Immunogen Sequence: QFDSFLESTRTYLGVE (a.a. 103-118)
Entrez Gene ID: 575
Antibody Registry ID: AB_2814782
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CPTC-BAI1-2 was deposited to the DSHB by Clinical Proteomics Technologies for Cancer (DSHB Hybridoma Product CPTC-BAI1-2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).