Antigen: BRCA1 interacting protein C-terminal helicase 1
Hybridoma Cells Available:
Antigen Species: Human
Depositor: Clinical Proteomics Technologies for Cancer
Host Species: mouse
Depositors Institution: National Cancer Institute
Positive Tested Species Reactivity: Human
Depositors Notes: This antigen is also known as Fanconi anemia group J protein.
Antigen Molecular Weight: 140.8 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: Synthetic Peptide
Alternate Gene Names: BRCA1 Interacting Protein C-Terminal Helicase 1; BACH1; FANCJ; BRCA1/BRCA2-Associated Helicase 1; ATP-Dependent RNA Helicase BRIP1; BRCA1-Associated C-Terminal Helicase 1; OF; BRCA1-Binding Helicase-Like Protein; Fanconi Anemia Group J Protein; EC 126.96.36.199; BRCA1-Interacting Protein 1; RCA1-Interacting Protein C-Terminal Helicase 1; Protein FACJ; EC 3.6.1
Alternate Antibody Name:
Alternate Antigen Name:
Myeloma Strain: P3x63Ag8.653
Epitope Location or Sequence:
Uniprot ID: Q9BX63
Immunogen Sequence: KATPELGSSENSASp(S)PPRF
Antibody Registry ID: AB_2617226
Additional Information: RRID:AB_2617226
Recommended Applications: ELISA
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CPTC-BRIP1-1 was deposited to the DSHB by Clinical Proteomics Technologies for Cancer (DSHB Hybridoma Product CPTC-BRIP1-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).