CPTC-CD44-2

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$45.00
SKU: CPTC-CD44-2-s
View product citations for antibody CPTC-CD44-2 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: CD44 molecule (Indian blood group) Peptide 2
Available to For-Profits: Yes
Antigen Species: Human
Hybridoma Cells Available (Non-Profit): No
Isotype: Rabbit IgG
Depositor: Paulovich, A.P.
Host Species: rabbit
Antigen Sequence:
Positive Tested Species Reactivity: Human
Depositors Institution: Fred Hutchinson Cancer Research Center
Antigen Molecular Weight: 81.5 kDa
Depositors Notes: CD44 is involved in homeotypic interactions, cell adhesion and migration. It is a cell-surface receptor for a number of ECM proteins.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Synthetic peptide
Gene: CD44
Alternate Antibody Name:
Alternate Gene Names: CDW44, CSPG8, ECMR-III, HCELL, HUTCH-I, IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: 240E-W2
Epitope Mapped: Yes
Uniprot ID: P16070 
Epitope Location or Sequence: YGFIEGHVVIPR
Entrez Gene ID: 960 
Immunogen Sequence: YGFIEGHVVIPR
Antibody Registry ID: AB_2889957 
Recommended Applications:  
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CPTC-CD44-2 was deposited to the DSHB by Paulovich, A.P. (DSHB Hybridoma Product CPTC-CD44-2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

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