Antigen: Cell cycle and apoptosis regulator 2
Hybridoma Cells Available: No
Antigen Species: Human
Depositor: Clinical Proteomics Technologies for Cancer
Depositors Institution: National Cancer Institute
Host Species: mouse
Depositors Notes: This antibody recognizes a core protein of the DBIRD complex.
Positive Tested Species Reactivity: Human
Human Protein Atlas: http://www.proteinatlas.org/ENSG00000158941-CCAR2/antibody
Antigen Molecular Weight: 48.3 kDa
Immunogen: Recombinant Full Length Protein
Alternate Gene Names: DBC1; DBC-1; NET35; P30 DBC; DBIRD Complex Subunit KIAA1967; Deleted In Breast Cancer 1; P30 DBC Protein; P30DBC; DBC.1; Deleted In Breast Cancer Gene 1 Protein
Myeloma Strain: P3x63Ag8.653
Uniprot ID: Q8N163
Epitope Mapped: No
Entrez Gene ID: 57805
Immunogen Sequence: Full length sequence
Antibody Registry ID: AB_2617284
Additional Characterization: https://antibodies.cancer.gov/detail/CPTC-KIAA1967-1
Recommended Applications: ELISA, Microarray, Western Blot
Additional Information: RRID:AB_2617284
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CPTC-KIAA1967-1 was deposited to the DSHB by Clinical Proteomics Technologies for Cancer (DSHB Hybridoma Product CPTC-KIAA1967-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).