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View product citations for antibody CPTC-KLHDC8A-1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Kelch Domain Containing 8A Peptide 1
Hybridoma Cells Available:
Antigen Species: Human
Depositor: Clinical Proteomics Technologies for Cancer
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: National Cancer Institute
Positive Tested Species Reactivity: Human
Depositors Notes: This antibody recognizes a protein that is upregulated in most carcinomas.
Antigen Molecular Weight: 38.8 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Immunogen: Synthetic Peptide
Alternate Gene Names: Kelch Domain Containing 8A; Substitute For Delta-EGFR Expression 1; S-delta-E1; Kelch Domain-Containing Protein 8A
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: P3x63Ag8.653
Epitope Location or Sequence: a.a. 222-244
Uniprot ID: Q8IYD2 
Entrez Gene ID: 55220 
Additional Characterization: Link 
Antibody Registry ID: AB_2617286 
Additional Information: RRID:AB_2617286
Recommended Applications: Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CPTC-KLHDC8A-1 was deposited to the DSHB by Clinical Proteomics Technologies for Cancer (DSHB Hybridoma Product CPTC-KLHDC8A-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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