Antigen: Membrane Spanning 4-Domains A1 Peptide 1
Available to For-Profits: Yes
Antigen Species: Human
Hybridoma Cells Available (Non-Profit): No
Isotype: Rabbit IgG
Depositor: Paulovich, A.P.
Host Species: rabbit
Positive Tested Species Reactivity: Human
Depositors Institution: Fred Hutchinson Cancer Research Center
Antigen Molecular Weight: 33.0 kDa
Depositors Notes: This antigen is also known as B-lymphocyte antigen CD20.
Immunogen: Synthetic Peptide
Alternate Antibody Name:
Alternate Gene Names: CD20, B1, Bp35, CVID5, FMC7, LEU-16, S7
Alternate Antigen Name:
Myeloma Strain: P3x63Ag8.653
Epitope Mapped: Yes
Uniprot ID: P11836
Epitope Location or Sequence: a.a. 244-257
Entrez Gene ID: 931
Immunogen Sequence: EEVVGLTETSSQPK
Additional Characterization: https://antibodies.cancer.gov/detail/MembraneSpanning4-DomainsA1Peptide1
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CPTC-MS4A1-1 was deposited to the DSHB by Paulovich, A.P. (DSHB Hybridoma Product CPTC-MS4A1-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.