Antigen: Neurofibromin 1
Hybridoma Cells Available: No
Antigen Species: Human
Depositor: Clinical Proteomics Technologies for Cancer
Host Species: mouse
Depositors Institution: National Cancer Institute
Positive Tested Species Reactivity: Human
Depositors Notes: Neurofibromin is a negative regulator of Ras signaling pathway. Mutations in NF1 result in variations of the disease neurofibromatosis.
Predicted Species Reactivity:
Immunogen: Recombinant protein
Alternate Gene Names: NFNS, VRNF, WSS
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3x63Ag8.653
Epitope Location or Sequence:
Uniprot ID: P21359
Immunogen Sequence: Full length sequence
Entrez Gene ID: 4763
Additional Characterization: https://antibodies.cancer.gov/detail/CPTC-NF1-1#CPTC-NF1-1
Antibody Registry ID: AB_2920914
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CPTC-NF1-1 was deposited to the DSHB by Clinical Proteomics Technologies for Cancer (DSHB Hybridoma Product CPTC-NF1-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.