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SKU: CPTC-S100A2-1-s
View product citations for antibody CPTC-S100A2-1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: S100 calcium binding protein A2
Hybridoma Cells Available: No
Antigen Species: Human
Depositor: Clinical Proteomics Technologies for Cancer
Isotype: MIgG2b
Depositors Institution: National Cancer Institute
Host Species: mouse
Depositors Notes: This antibody has been validated by the Human Protein Atlas. http://www.proteinatlas.org/ENSG000 00196754?S100A2/antibody.
Positive Tested Species Reactivity: Human
Gene: S100A2
Immunogen: Recombinant Full Length Protein
Alternate Gene Names: CAN19; MGC111539; S100L
Myeloma Strain: P3x63Ag8.653
Clonality: Monoclonal
Uniprot ID: P29034 
Epitope Mapped: No
Entrez Gene ID: 6273 
Antibody Registry ID: AB_2617335 
Additional Information: Antibody has been characterized only by ELISA or western blot. RRID:AB_2617335
Recommended Applications: ELISA, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CPTC-S100A2-1 was deposited to the DSHB by Clinical Proteomics Technologies for Cancer (DSHB Hybridoma Product CPTC-S100A2-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

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