Antigen: DNA topoisomerase I, mitochondrial
Antigen Species: Human
Depositors Institution: National Cancer Institute
Host Species: mouse
Positive Tested Species Reactivity: Human
Immunogen Sequence: Full length sequence
Antigen Molecular Weight: 69.8 kDa
Additional Characterization: https://antibodies.cancer.gov/detail/CPTC-TOP1MT-3#CPTC-TOP1MT-3
Immunogen: E.coli expressed recombinant
Additional Information: RRID: AB_2617397
Myeloma Strain: P3x63Ag8.653
Uniprot ID: Q969P6
Entrez Gene ID: 116447
Antibody Registry ID: AB_2617397
Recommended Applications: ELISA, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CPTC-TOP1MT-3 was deposited to the DSHB by CPTC (DSHB Hybridoma Product CPTC-TOP1MT-3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).