CT3

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$45.00 to $425.00
SKU: CT3
View product citations for antibody CT3 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: troponin T (cardiac/slow)
Available to For-Profits: Yes
Antigen Species: Bovine
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG2a
Depositor: Lin, J.J.-C.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Broad species, Chicken, Fish, Human, Mouse, Rabbit, Rat, Xenopus
Depositors Institution: University of Iowa
Antigen Molecular Weight: Predicted (cTnT): 34 kDa; Apparent (cTnT): 43kDa
Depositors Notes: CT3 was initially published in: Jin, J., Lin, J.-C., and Lin, J.J.-C. (1990). Troponin T isoform switching during heart development. Ann. NY Acad. Sci. 588, 393-396.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Purified cardiac troponin T
Gene: TNNT2
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: NS1
Epitope Mapped: Yes
Uniprot ID: P13789 
Epitope Location or Sequence: aa 94-178
Entrez Gene ID: 286816 
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_528495 
Additional Characterization:  
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
Additional Information: CT3 recognizes the embryonic and adult cardiac isforms [PMID: 2358124]. CT3 cross-react with slow skeletal muscle TnT but doesn't recognize fast skeletal muscle TnT [PMID: 12732643].
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CT3 was deposited to the DSHB by Lin, J.J.-C. (DSHB Hybridoma Product CT3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

16 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References

    Regulation of vertebrate embryogenesis by the exon junction complex core component Eif4a3.
    Weinstein DC
    Developmental dynamics : an official publication of the American Association of Anatomists 239.7 (2010 Jul): 1977-87.

    Truncation by Glu180 nonsense mutation results in complete loss of slow skeletal muscle troponin T in a lethal nemaline myopathy.
    Crawford TO
    The Journal of biological chemistry 278.28 (2003 Jul 11): 26159-65.

    CUG-BP, Elav-like family member 1 (CELF1) is required for normal myofibrillogenesis, morphogenesis, and contractile function in the embryonic heart.
    Ladd AN
    Developmental dynamics : an official publication of the American Association of Anatomists 245.8 (2016 Aug): 854-73.

    CUG-BP, Elav-like family member 1 (CELF1) is required for normal myofibrillogenesis, morphogenesis, and contractile function in the embryonic heart.
    Ladd AN
    Developmental dynamics : an official publication of the American Association of Anatomists 245.8 (2016 Aug): 854-73.

    FACS References

    Heterogeneity of Mesp1+ mesoderm revealed by single-cell RNA-seq.
    Kyba M
    Biochemical and biophysical research communications 474.3 (2016 Jun 3): 469-475.

    Epitope Map References

    Localization of the two tropomyosin-binding sites of troponin T.
    Chong SM
    Archives of biochemistry and biophysics 500.2 (2010 Aug 15): 144-50.

    All References

    Regulation of vertebrate embryogenesis by the exon junction complex core component Eif4a3.
    Weinstein DC
    Developmental dynamics : an official publication of the American Association of Anatomists 239.7 (2010 Jul): 1977-87.

    Truncation by Glu180 nonsense mutation results in complete loss of slow skeletal muscle troponin T in a lethal nemaline myopathy.
    Crawford TO
    The Journal of biological chemistry 278.28 (2003 Jul 11): 26159-65.

    CUG-BP, Elav-like family member 1 (CELF1) is required for normal myofibrillogenesis, morphogenesis, and contractile function in the embryonic heart.
    Ladd AN
    Developmental dynamics : an official publication of the American Association of Anatomists 245.8 (2016 Aug): 854-73.

    Precocious appearance of cardiac troponin T pre-mRNAs during early avian embryonic skeletal muscle development in ovo.
    Solursh M
    Developmental biology 140.1 (1990 Jul): 73-82.

    Forced expression and assembly of rat cardiac troponin T isoforms in cultured muscle and nonmuscle cells.
    Lin JJ
    Journal of muscle research and cell motility 14.6 (1993 Dec): 619-32.

    Confocal imaging of early heart development in Xenopus laevis.
    Weeks DL
    Developmental biology 218.1 (2000 Feb 1): 64-73.

    Pitx2c attenuation results in cardiac defects and abnormalities of intestinal orientation in developing Xenopus laevis.
    Weeks DL
    Developmental biology 262.2 (2003 Oct 15): 268-81.

    Compensatory growth of healthy cardiac cells in the presence of diseased cells restores tissue homeostasis during heart development.
    Cox TC
    Developmental cell 15.4 (2008 Oct): 521-33.

    Requirement of a novel gene, Xin, in cardiac morphogenesis.
    Lin JJ
    Development (Cambridge, England) 126.6 (1999 Mar): 1281-94.

    Insulin-like growth factor (IGF) binding protein-5 blocks skeletal muscle differentiation by inhibiting IGF actions.
    Rotwein P
    Molecular endocrinology (Baltimore, Md.) 22.1 (2008 Jan): 206-15.

    Heart regeneration in adult <i>Xenopus tropicalis</i> after apical resection.
    Cai D
    Cell & bioscience 7. (2017): 70.

    Muscle specific fragile X related protein 1 isoforms are sequestered in the nucleus of undifferentiated myoblast.
    Khandjian EW
    BMC genetics 1. (2000): 4.

    Alternative RNA splicing-generated cardiac troponin T isoform switching: a non-heart-restricted genetic programming synchronized in developing cardiac and skeletal muscles.
    Jin JP
    Biochemical and biophysical research communications 225.3 (1996 Aug 23): 883-9.

    Conformational modulation of slow skeletal muscle troponin T by an NH(2)-terminal metal-binding extension.
    Huang QQ
    American journal of physiology. Cell physiology 279.4 (2000 Oct): C1067-77.

    Localization of the two tropomyosin-binding sites of troponin T.
    Chong SM
    Archives of biochemistry and biophysics 500.2 (2010 Aug 15): 144-50.

    Heterogeneity of Mesp1+ mesoderm revealed by single-cell RNA-seq.
    Kyba M
    Biochemical and biophysical research communications 474.3 (2016 Jun 3): 469-475.

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