CaF1-5C3

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$40.00
SKU: CaF1-5C3
View product citations for antibody CaF1-5C3 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: ATPase, Ca(+2) fast twitch SR (avian) / SERCA1
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Fambrough, D.M.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Johns Hopkins School of Medicine
Positive Tested Species Reactivity: Chicken, Duck
Depositors Notes: Immunoblotting: very good. Immunohistochemistry: good on cryosections and methanol and formaldehyde fixed tissues.
Antigen Molecular Weight: 110 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: ATP2A1
Immunogen: Sarcoplasmic reticulum membrane vesicles preparation from adult chicken pectoralis major muscle.
Alternate Gene Names: SERCA1
Alternate Antibody Name: CAF3-5C3; 5C3; CAF3
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: SP2/0 Ag8
Epitope Location or Sequence:
Uniprot ID: P13585 
Immunogen Sequence: Total protein
Entrez Gene ID: 396528 
Additional Characterization:
Antibody Registry ID: AB_531813 
Additional Information:
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CaF1-5C3 was deposited to the DSHB by Fambrough, D.M. (DSHB Hybridoma Product CaF1-5C3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

18 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • All References
  • Initial Publication
    IF References

    Expression of fast and slow isoforms of the Ca2+-ATPase in developing chick skeletal muscle.
    Fambrough DM
    Developmental biology 124.2 (1987 Dec): 490-503.

    Expression of the sarco/endoplasmic Ca(2+)-ATPase, SERCA1a, in fibroblasts induces the formation of organelle membrane arrays.
    Karin NJ
    Experimental cell research 292.1 (2004 Jan 1): 78-88.

    Characterization of WWP1 protein expression in skeletal muscle of muscular dystrophy chickens.
    Takeda S
    Journal of biochemistry 159.2 (2016 Feb): 171-9.

    Cell-specific promoter in adenovirus vector for transgenic expression of SERCA1 ATPase in cardiac myocytes.
    Ordahl CP
    The American journal of physiology 274.3 (1998 Mar): C645-53.

    Expression of avian Ca2+-ATPase in cultured mouse myogenic cells.
    Fambrough DM
    Molecular and cellular biology 9.5 (1989 May): 1978-86.

    Direct involvement of intracellular Ca2+ transport ATPase in the development of thapsigargin resistance by Chinese hamster lung fibroblasts.
    Inesi G
    The Journal of biological chemistry 270.20 (1995 May 19): 12140-6.

    Cell-specific expression of SERCA, the exogenous Ca2+ transport ATPase, in cardiac myocytes.
    Inesi G
    American journal of physiology. Cell physiology 286.3 (2004 Mar): C556-64.

    Exogenous Ca2+-ATPase isoform effects on Ca2+ transients of embryonic chicken and neonatal rat cardiac myocytes.
    Klein MG
    The Journal of physiology 528 Pt 1. (2000 Oct 1): 53-63.

    Nucleotide sequences of avian cardiac and brain SR/ER Ca(2+)-ATPases and functional comparisons with fast twitch Ca(2+)-ATPase. Calcium affinities and inhibitor effects.
    Fambrough DM
    The Journal of biological chemistry 266.24 (1991 Aug 25): 16050-5.

    WB References

    Expression of fast and slow isoforms of the Ca2+-ATPase in developing chick skeletal muscle.
    Fambrough DM
    Developmental biology 124.2 (1987 Dec): 490-503.

    Expression of the sarco/endoplasmic Ca(2+)-ATPase, SERCA1a, in fibroblasts induces the formation of organelle membrane arrays.
    Karin NJ
    Experimental cell research 292.1 (2004 Jan 1): 78-88.

    Cell-specific promoter in adenovirus vector for transgenic expression of SERCA1 ATPase in cardiac myocytes.
    Ordahl CP
    The American journal of physiology 274.3 (1998 Mar): C645-53.

    Expression of avian Ca2+-ATPase in cultured mouse myogenic cells.
    Fambrough DM
    Molecular and cellular biology 9.5 (1989 May): 1978-86.

    Detailed characterization of the cooperative mechanism of Ca(2+) binding and catalytic activation in the Ca(2+) transport (SERCA) ATPase.
    Toyoshima C
    Biochemistry 39.30 (2000 Aug 1): 8758-67.

    Substrate-induced conformational fit and headpiece closure in the Ca2+ATPase (SERCA).
    Toyoshima C
    The Journal of biological chemistry 278.31 (2003 Aug 1): 28938-43.

    Ca(2+)-dependent and thapsigargin-inhibited phosphorylation of Na+,K(+)-ATPase catalytic domain following chimeric recombination with Ca(2+)-ATPase.
    Fambrough DM
    The Journal of biological chemistry 268.28 (1993 Oct 5): 21185-92.

    Direct involvement of intracellular Ca2+ transport ATPase in the development of thapsigargin resistance by Chinese hamster lung fibroblasts.
    Inesi G
    The Journal of biological chemistry 270.20 (1995 May 19): 12140-6.

    Cell-specific expression of SERCA, the exogenous Ca2+ transport ATPase, in cardiac myocytes.
    Inesi G
    American journal of physiology. Cell physiology 286.3 (2004 Mar): C556-64.

    Exogenous Ca2+-ATPase isoform effects on Ca2+ transients of embryonic chicken and neonatal rat cardiac myocytes.
    Klein MG
    The Journal of physiology 528 Pt 1. (2000 Oct 1): 53-63.

    Dependence of exogenous SERCA gene expression on coxsackie adenovirus receptor levels in neonatal and adult cardiac myocytes.
    Inesi G
    Archives of biochemistry and biophysics 415.2 (2003 Jul 15): 178-83.

    Expression of sarcoplasmic reticulum Ca2+ transport proteins in cold-acclimating ducklings.
    Meissner G
    The American journal of physiology 269.4 Pt 1 (1995 Oct): C955-60.

    Nucleotide sequences of avian cardiac and brain SR/ER Ca(2+)-ATPases and functional comparisons with fast twitch Ca(2+)-ATPase. Calcium affinities and inhibitor effects.
    Fambrough DM
    The Journal of biological chemistry 266.24 (1991 Aug 25): 16050-5.

    IP References
    All References

    Expression of fast and slow isoforms of the Ca2+-ATPase in developing chick skeletal muscle.
    Fambrough DM
    Developmental biology 124.2 (1987 Dec): 490-503.

    Expression of the sarco/endoplasmic Ca(2+)-ATPase, SERCA1a, in fibroblasts induces the formation of organelle membrane arrays.
    Karin NJ
    Experimental cell research 292.1 (2004 Jan 1): 78-88.

    Characterization of WWP1 protein expression in skeletal muscle of muscular dystrophy chickens.
    Takeda S
    Journal of biochemistry 159.2 (2016 Feb): 171-9.

    Cell-specific promoter in adenovirus vector for transgenic expression of SERCA1 ATPase in cardiac myocytes.
    Ordahl CP
    The American journal of physiology 274.3 (1998 Mar): C645-53.

    Expression of avian Ca2+-ATPase in cultured mouse myogenic cells.
    Fambrough DM
    Molecular and cellular biology 9.5 (1989 May): 1978-86.

    Direct involvement of intracellular Ca2+ transport ATPase in the development of thapsigargin resistance by Chinese hamster lung fibroblasts.
    Inesi G
    The Journal of biological chemistry 270.20 (1995 May 19): 12140-6.

    Cell-specific expression of SERCA, the exogenous Ca2+ transport ATPase, in cardiac myocytes.
    Inesi G
    American journal of physiology. Cell physiology 286.3 (2004 Mar): C556-64.

    Exogenous Ca2+-ATPase isoform effects on Ca2+ transients of embryonic chicken and neonatal rat cardiac myocytes.
    Klein MG
    The Journal of physiology 528 Pt 1. (2000 Oct 1): 53-63.

    Nucleotide sequences of avian cardiac and brain SR/ER Ca(2+)-ATPases and functional comparisons with fast twitch Ca(2+)-ATPase. Calcium affinities and inhibitor effects.
    Fambrough DM
    The Journal of biological chemistry 266.24 (1991 Aug 25): 16050-5.

    Detailed characterization of the cooperative mechanism of Ca(2+) binding and catalytic activation in the Ca(2+) transport (SERCA) ATPase.
    Toyoshima C
    Biochemistry 39.30 (2000 Aug 1): 8758-67.

    Substrate-induced conformational fit and headpiece closure in the Ca2+ATPase (SERCA).
    Toyoshima C
    The Journal of biological chemistry 278.31 (2003 Aug 1): 28938-43.

    Ca(2+)-dependent and thapsigargin-inhibited phosphorylation of Na+,K(+)-ATPase catalytic domain following chimeric recombination with Ca(2+)-ATPase.
    Fambrough DM
    The Journal of biological chemistry 268.28 (1993 Oct 5): 21185-92.

    Dependence of exogenous SERCA gene expression on coxsackie adenovirus receptor levels in neonatal and adult cardiac myocytes.
    Inesi G
    Archives of biochemistry and biophysics 415.2 (2003 Jul 15): 178-83.

    Expression of sarcoplasmic reticulum Ca2+ transport proteins in cold-acclimating ducklings.
    Meissner G
    The American journal of physiology 269.4 Pt 1 (1995 Oct): C955-60.

    26 amino acids of an extracellular domain of the Na,K-ATPase alpha-subunit are sufficient for assembly with the Na,K-ATPase beta-subunit.
    Fambrough DM
    The Journal of biological chemistry 269.11 (1994 Mar 18): 8255-9.

    Failure to make normal alpha ryanodine receptor is an early event associated with the crooked neck dwarf (cn) mutation in chicken.
    Talvenheimo J
    Developmental dynamics : an official publication of the American Association of Anatomists 197.3 (1993 Jul): 169-88.

    Crooked neck dwarf (cn) mutant chicken skeletal muscle cells in low density primary cultures fail to express normal alpha ryanodine receptor and exhibit a partial mutant phenotype.
    Sutko JL
    Developmental dynamics : an official publication of the American Association of Anatomists 197.3 (1993 Jul): 189-202.

    Ca2+ occlusion and gating function of Glu309 in the ADP-fluoroaluminate analog of the Ca2+-ATPase phosphoenzyme intermediate.
    Xu C
    The Journal of biological chemistry 279.30 (2004 Jul 23): 31629-37.

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