CaS/C1 or 3H2

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SKU: CaS/C1 or 3H2
View product citations for antibody CaS/C1 or 3H2 on CiteAb

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Available: 29

DSHB Data Sheet
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Catalog Fields

Antigen: ATPase, Ca(+2) slow twitch/cardiac SR (avian), SERCA2
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Fambrough, D.M.
Isotype: MIgG1, kappa light chain
Depositors Institution: Johns Hopkins School of Medicine
Host Species: mouse
Depositors Notes: Immunoblotting: very good. Immunohistochemistry: good on cryosections and formaldehyde fixed tissues. The epitope is destroyed with methanol and glutaraldehyde.
Positive Tested Species Reactivity: Chicken, Duck
Gene: ATP2A2
Antigen Molecular Weight: 115 kDa
Alternate Gene Names: SERCA2
Immunogen: Sarcoplasmic reticulum membrane vesicles preparation from adult chicken hearts.
Clonality: Monoclonal
Alternate Antibody Name: Cas-3H2; 3H2
Epitope Mapped: No
Myeloma Strain: SP2/0 Ag8
Immunogen Sequence: Total protein
Uniprot ID: Q03669 
Entrez Gene ID: 396446 
Antibody Registry ID: AB_531764 
Recommended Applications: Immunofluorescence, Western Blot
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These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CaS/C1 or 3H2 was deposited to the DSHB by Fambrough, D.M. (DSHB Hybridoma Product CaS/C1 or 3H2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

15 References

  • Initial Publication
  • IF References
  • WB References
  • All References
    • Initial Publication

    Expression of fast and slow isoforms of the Ca2+-ATPase in developing chick skeletal muscle.
    Fambrough DM
    Developmental biology 124.2 (1987 Dec): 490-503.

    IF References

    Expression of fast and slow isoforms of the Ca2+-ATPase in developing chick skeletal muscle.
    Fambrough DM
    Developmental biology 124.2 (1987 Dec): 490-503.

    Characterization of WWP1 protein expression in skeletal muscle of muscular dystrophy chickens.
    Takeda S
    Journal of biochemistry 159.2 (2016 Feb): 171-9.

    Sarco(endo)plasmic reticulum Ca2+ ATPase (SERCA) gene silencing and remodeling of the Ca2+ signaling mechanism in cardiac myocytes.
    Inesi G
    Proceedings of the National Academy of Sciences of the United States of America 101.47 (2004 Nov 23): 16683-8.

    Cell-specific expression of SERCA, the exogenous Ca2+ transport ATPase, in cardiac myocytes.
    Inesi G
    American journal of physiology. Cell physiology 286.3 (2004 Mar): C556-64.

    Exogenous Ca2+-ATPase isoform effects on Ca2+ transients of embryonic chicken and neonatal rat cardiac myocytes.
    Klein MG
    The Journal of physiology 528 Pt 1. (2000 Oct 1): 53-63.

    Nucleotide sequences of avian cardiac and brain SR/ER Ca(2+)-ATPases and functional comparisons with fast twitch Ca(2+)-ATPase. Calcium affinities and inhibitor effects.
    Fambrough DM
    The Journal of biological chemistry 266.24 (1991 Aug 25): 16050-5.

    WB References

    Expression of fast and slow isoforms of the Ca2+-ATPase in developing chick skeletal muscle.
    Fambrough DM
    Developmental biology 124.2 (1987 Dec): 490-503.

    Cell-specific promoter in adenovirus vector for transgenic expression of SERCA1 ATPase in cardiac myocytes.
    Ordahl CP
    The American journal of physiology 274.3 (1998 Mar): C645-53.

    Ca(2+)-dependent and thapsigargin-inhibited phosphorylation of Na+,K(+)-ATPase catalytic domain following chimeric recombination with Ca(2+)-ATPase.
    Fambrough DM
    The Journal of biological chemistry 268.28 (1993 Oct 5): 21185-92.

    Exogenous Ca2+-ATPase isoform effects on Ca2+ transients of embryonic chicken and neonatal rat cardiac myocytes.
    Klein MG
    The Journal of physiology 528 Pt 1. (2000 Oct 1): 53-63.

    Dependence of exogenous SERCA gene expression on coxsackie adenovirus receptor levels in neonatal and adult cardiac myocytes.
    Inesi G
    Archives of biochemistry and biophysics 415.2 (2003 Jul 15): 178-83.

    The alternative carboxyl termini of avian cardiac and brain sarcoplasmic reticulum/endoplasmic reticulum Ca(2+)-ATPases are on opposite sides of the membrane.
    Fambrough DM
    The Journal of biological chemistry 267.13 (1992 May 5): 9321-5.

    Expression of sarcoplasmic reticulum Ca2+ transport proteins in cold-acclimating ducklings.
    Meissner G
    The American journal of physiology 269.4 Pt 1 (1995 Oct): C955-60.

    Nucleotide sequences of avian cardiac and brain SR/ER Ca(2+)-ATPases and functional comparisons with fast twitch Ca(2+)-ATPase. Calcium affinities and inhibitor effects.
    Fambrough DM
    The Journal of biological chemistry 266.24 (1991 Aug 25): 16050-5.

    All References

    Expression of fast and slow isoforms of the Ca2+-ATPase in developing chick skeletal muscle.
    Fambrough DM
    Developmental biology 124.2 (1987 Dec): 490-503.

    Characterization of WWP1 protein expression in skeletal muscle of muscular dystrophy chickens.
    Takeda S
    Journal of biochemistry 159.2 (2016 Feb): 171-9.

    Sarco(endo)plasmic reticulum Ca2+ ATPase (SERCA) gene silencing and remodeling of the Ca2+ signaling mechanism in cardiac myocytes.
    Inesi G
    Proceedings of the National Academy of Sciences of the United States of America 101.47 (2004 Nov 23): 16683-8.

    Cell-specific expression of SERCA, the exogenous Ca2+ transport ATPase, in cardiac myocytes.
    Inesi G
    American journal of physiology. Cell physiology 286.3 (2004 Mar): C556-64.

    Exogenous Ca2+-ATPase isoform effects on Ca2+ transients of embryonic chicken and neonatal rat cardiac myocytes.
    Klein MG
    The Journal of physiology 528 Pt 1. (2000 Oct 1): 53-63.

    Nucleotide sequences of avian cardiac and brain SR/ER Ca(2+)-ATPases and functional comparisons with fast twitch Ca(2+)-ATPase. Calcium affinities and inhibitor effects.
    Fambrough DM
    The Journal of biological chemistry 266.24 (1991 Aug 25): 16050-5.

    Cell-specific promoter in adenovirus vector for transgenic expression of SERCA1 ATPase in cardiac myocytes.
    Ordahl CP
    The American journal of physiology 274.3 (1998 Mar): C645-53.

    Ca(2+)-dependent and thapsigargin-inhibited phosphorylation of Na+,K(+)-ATPase catalytic domain following chimeric recombination with Ca(2+)-ATPase.
    Fambrough DM
    The Journal of biological chemistry 268.28 (1993 Oct 5): 21185-92.

    Dependence of exogenous SERCA gene expression on coxsackie adenovirus receptor levels in neonatal and adult cardiac myocytes.
    Inesi G
    Archives of biochemistry and biophysics 415.2 (2003 Jul 15): 178-83.

    The alternative carboxyl termini of avian cardiac and brain sarcoplasmic reticulum/endoplasmic reticulum Ca(2+)-ATPases are on opposite sides of the membrane.
    Fambrough DM
    The Journal of biological chemistry 267.13 (1992 May 5): 9321-5.

    Expression of sarcoplasmic reticulum Ca2+ transport proteins in cold-acclimating ducklings.
    Meissner G
    The American journal of physiology 269.4 Pt 1 (1995 Oct): C955-60.

    Failure to make normal alpha ryanodine receptor is an early event associated with the crooked neck dwarf (cn) mutation in chicken.
    Talvenheimo J
    Developmental dynamics : an official publication of the American Association of Anatomists 197.3 (1993 Jul): 169-88.

    Tyr-167/Trp-168 in type 1/3 inositol 1,4,5-trisphosphate receptor mediates functional coupling between ligand binding and channel opening.
    Mikoshiba K
    The Journal of biological chemistry 285.46 (2010 Nov 12): 36081-91.

    Crooked neck dwarf (cn) mutant chicken skeletal muscle cells in low density primary cultures fail to express normal alpha ryanodine receptor and exhibit a partial mutant phenotype.
    Sutko JL
    Developmental dynamics : an official publication of the American Association of Anatomists 197.3 (1993 Jul): 189-202.

    Expression of avian Ca2+-ATPase in cultured mouse myogenic cells.
    Fambrough DM
    Molecular and cellular biology 9.5 (1989 May): 1978-86.

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