CaS/C1 or 3H2
Catalog Fields
Antigen: ATPase, Ca(+2) slow twitch/cardiac SR (avian), SERCA2
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Fambrough, D.M.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Johns Hopkins School of Medicine
Positive Tested Species Reactivity: Chicken, Duck
Depositors Notes: Immunoblotting: very good. Immunohistochemistry: good on cryosections and formaldehyde fixed tissues. The epitope is destroyed with methanol and glutaraldehyde.
Predicted Species Reactivity:
Gene: ATP2A2
Immunogen: Sarcoplasmic reticulum membrane vesicles preparation from adult chicken hearts.
Alternate Gene Names: SERCA2
Alternate Antibody Name: Cas-3H2; 3H2
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0 Ag8
Epitope Location or Sequence:
Uniprot ID: Q03669
Immunogen Sequence: Total protein
Entrez Gene ID: 396446
Antibody Registry ID: AB_531764
Additional Information:
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CaS/C1 or 3H2 was deposited to the DSHB by Fambrough, D.M. (DSHB Hybridoma Product CaS/C1 or 3H2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
15 References
Initial Publication
Expression of fast and slow isoforms of the Ca2+-ATPase in developing chick skeletal muscle.
Fambrough DM
Developmental biology 124.2 (1987 Dec): 490-503.