Cutoff 9D5-8C9

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$40.00
SKU: Cutoff 9D5-8C9
View product citations for antibody Cutoff 9D5-8C9 on CiteAb

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Available: 40

DSHB Data Sheet

Catalog Fields

Antigen: Cutoff protein
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Schupbach, T.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Princeton University - Princeton, NJ
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: 44.8 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: cuff
Immunogen: Recombinant Cutoff protein
Alternate Gene Names: Dmel_CG13190
Alternate Antibody Name:
Clonality:
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID: Q9V629 
Immunogen Sequence:
Entrez Gene ID: 36269 
Additional Characterization:  
Antibody Registry ID: AB_10572165 
Additional Information: RRID: AB_10572165 FBgn0260932.
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Cutoff 9D5-8C9 was deposited to the DSHB by Schupbach, T. (DSHB Hybridoma Product Cutoff 9D5-8C9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

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  • Initial Publication
    IF References
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