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$45.00 to $425.00
SKU: D-B3(act)
View product citations for antibody D-B3(act) on CiteAb

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Available: 32

DSHB Data Sheet

Catalog Fields

Antigen: TGFbeta3, functional, active domain
Available to For-Profits: Yes
Antigen Species: chicken
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgM
Depositor: Runyan, R.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Chicken, Lizard, Mammal, Planaria
Depositors Institution: University of Arizona
Antigen Molecular Weight: Predicted: 45 kDa, 32 kDa (latent peptide), 13 kDa (TGFbate3 active peptide)
Depositors Notes: Stains chick embryo heart.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Recombinant GST/TGFbeta3 fusion protein (one of three boosts with active domain of TGFbeta3)
Gene: TGFB3
Alternate Antibody Name: D-B3
Alternate Gene Names: TGF-beta3
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: NS-1
Epitope Mapped: Yes
Uniprot ID: P16047 
Epitope Location or Sequence: TGFbeta3 N-terminus (ALDTNY)
Entrez Gene ID: 396438 
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_531801 
Additional Characterization:  
Recommended Applications: ELISA, FFPE, Function Blocking, Immunofluorescence, Immunohistochemistry, Western Blot
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
D-B3(act) was deposited to the DSHB by Runyan, R. (DSHB Hybridoma Product D-B3(act))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

4 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • FFPE References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References

    Scar-free wound healing and regeneration following tail loss in the leopard gecko, Eublepharis macularius.
    Vickaryous MK
    Anatomical record (Hoboken, N.J. : 2007) 295.10 (2012 Oct): 1575-95.

    ELISA References
    FFPE References
    FB References
    Epitope Map References
    All References

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