D1-9

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$40.00
SKU: D1-9-s
View product citations for antibody D1-9 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Collagen type IX
Hybridoma Cells Available: No
Antigen Species: Bovine
Depositor: Ye, X.-J. / Terato, K.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Tennessee, Memphis
Positive Tested Species Reactivity: Bovine, Human, Rat
Depositors Notes:
Antigen Molecular Weight: 94.0 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: COL9A1
Immunogen: Purified collagen IX
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: Sp2/0-Ag14
Epitope Location or Sequence: COL1 helical domain (LMW)
Uniprot ID: F1MND9 
Immunogen Sequence: Full length protein
Entrez Gene ID: 282195 
Additional Characterization:  
Antibody Registry ID: AB_528169 
Additional Information: RRID:AB_528169
Recommended Applications: ELISA, FFPE, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
D1-9 was deposited to the DSHB by Ye, X.-J. / Terato, K. (DSHB Hybridoma Product D1-9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

7 References

  • Initial Publication
  • WB References
  • IHC References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication

    Monoclonal antibodies against bovine type IX collagen (LMW fragment): production, characterization, and use for immunohistochemical localization studies.
    Yoo TJ
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 39.3 (1991 Mar): 265-71.

    WB References

    Monoclonal antibodies against bovine type IX collagen (LMW fragment): production, characterization, and use for immunohistochemical localization studies.
    Yoo TJ
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 39.3 (1991 Mar): 265-71.

    IHC References
    ELISA References

    Monoclonal antibodies against bovine type IX collagen (LMW fragment): production, characterization, and use for immunohistochemical localization studies.
    Yoo TJ
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 39.3 (1991 Mar): 265-71.

    Epitope Map References

    Monoclonal antibodies against bovine type IX collagen (LMW fragment): production, characterization, and use for immunohistochemical localization studies.
    Yoo TJ
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 39.3 (1991 Mar): 265-71.

    All References

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