DA5F9-4
Catalog Fields
Antigen: Pinhead
Available to For-Profits: Yes
Antigen Species: Xenopus
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Alfandari, D.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Xenopus
Depositors Institution: University of Massachusetts, Amherst
Antigen Molecular Weight: 35.8 kDa
Depositors Notes:
Immunogen: Recombinant protein expressed in Hek293T
Gene: pnhd
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: SP2/0
Epitope Mapped: No
Uniprot ID: Q6VPR2
Epitope Location or Sequence:
Entrez Gene ID: 394459
Immunogen Sequence: Full Length
Recommended Applications: ELISA, Immunofluorescence, Western Blot
Additional Information: Please reference NIH Grant R24OD21485 in alll publications.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DA5F9-4 was deposited to the DSHB by Alfandari, D. (DSHB Hybridoma Product DA5F9-4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.