Hybridoma Cells Available: Yes
Antigen Species: Xenopus
Depositor: Alfandari, D.
Host Species: mouse
Depositors Institution: University of Massachusetts, Amherst
Positive Tested Species Reactivity: Xenopus
Predicted Species Reactivity:
Immunogen: Recombinant protein expressed in Hek293T
Alternate Gene Names:
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID: Q6VPR2
Immunogen Sequence: Full Length
Entrez Gene ID: 394459
Additional Information: Please reference NIH Grant R24OD21485 in alll publications.
Recommended Applications: ELISA, Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DA5F9-4 was deposited to the DSHB by Alfandari, D. (DSHB Hybridoma Product DA5F9-4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.