DCB32(pok13)

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$40.00
SKU: DCB32(pok13)
View product citations for antibody DCB32(pok13) on CiteAb

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Available: 32

DSHB Data Sheet

Catalog Fields

Antigen: Calbindin-32
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Buchner, E. / Hofbauer, A.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Wuerzburg - Germany
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Antigen Molecular Weight: 35.7 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Cbp53E
Immunogen: Recombinant calbindin-32
Alternate Gene Names: cbn, CG6702
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3X.63-Ag8.653
Epitope Location or Sequence:
Uniprot ID: P41044 
Immunogen Sequence: Full length protein
Entrez Gene ID: 36905 
Additional Characterization:  
Antibody Registry ID: AB_2617211 
Additional Information: RRID: AB_2617211 FBgn0004580.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DCB32(pok13) was deposited to the DSHB by Buchner, E. / Hofbauer, A. (DSHB Hybridoma Product DCB32(pok13))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication

    An invertebrate calcium-binding protein of the calbindin subfamily: protein structure, genomic organization, and expression pattern of the calbindin-32 gene of Drosophila.
    Schug B
    The Journal of neuroscience : the official journal of the Society for Neuroscience 13.5 (1993 May): 2186-98.

    IF References
    WB References

    An invertebrate calcium-binding protein of the calbindin subfamily: protein structure, genomic organization, and expression pattern of the calbindin-32 gene of Drosophila.
    Schug B
    The Journal of neuroscience : the official journal of the Society for Neuroscience 13.5 (1993 May): 2186-98.

    The Wuerzburg hybridoma library against Drosophila brain.
    Buchner E
    Journal of neurogenetics 23.1-2 (2009): 78-91.

    IHC References

    An invertebrate calcium-binding protein of the calbindin subfamily: protein structure, genomic organization, and expression pattern of the calbindin-32 gene of Drosophila.
    Schug B
    The Journal of neuroscience : the official journal of the Society for Neuroscience 13.5 (1993 May): 2186-98.

    The Wuerzburg hybridoma library against Drosophila brain.
    Buchner E
    Journal of neurogenetics 23.1-2 (2009): 78-91.

    All References

    An invertebrate calcium-binding protein of the calbindin subfamily: protein structure, genomic organization, and expression pattern of the calbindin-32 gene of Drosophila.
    Schug B
    The Journal of neuroscience : the official journal of the Society for Neuroscience 13.5 (1993 May): 2186-98.

    The Wuerzburg hybridoma library against Drosophila brain.
    Buchner E
    Journal of neurogenetics 23.1-2 (2009): 78-91.

    Drosophila Cbp53E Regulates Axon Growth at the Neuromuscular Junction.
    Tessier CR
    PloS one 10.7 (2015): e0132636.

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