Catalog Fields
Antigen: Calbindin-32
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Buchner, E. / Hofbauer, A.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Wuerzburg - Germany
Positive Tested Species Reactivity: Drosophila
Depositors Notes:
Predicted Species Reactivity:
Gene: Cbp53E
Immunogen: Recombinant calbindin-32
Alternate Gene Names: cbn, CG6702
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3X.63-Ag8.653
Epitope Location or Sequence:
Uniprot ID: P41044
Immunogen Sequence: Full length protein
Entrez Gene ID: 36905
Antibody Registry ID: AB_2617211
Additional Information: RRID: AB_2617211 FBgn0004580.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DCB32(pok13) was deposited to the DSHB by Buchner, E. / Hofbauer, A. (DSHB Hybridoma Product DCB32(pok13))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
3 References
Initial Publication
An invertebrate calcium-binding protein of the calbindin subfamily: protein structure, genomic organization, and expression pattern of the calbindin-32 gene of Drosophila.
Schug B
The Journal of neuroscience : the official journal of the Society for Neuroscience 13.5 (1993 May): 2186-98.