Hybridoma Cells Available: No
Antigen Species: bovine
Depositor: Poole, A.R.
Host Species: mouse
Depositors Institution: Shriners Hospital
Positive Tested Species Reactivity: Bovine
Depositors Notes: Immunohistology note: staining of bovine articular cartilage, epiphyseal matrix, outlined collagen fibrils in skin, anterior cruciate ligament, tendon, small arterial vessels in the dermis, extracellular matrix of the cornea, sclera and uveal tract. Antigen chemical properties: core protein epitope.
Predicted Species Reactivity:
Immunogen: decorin (dermatan sulfate proteoglycan DS-PS II)
Alternate Gene Names:
Alternate Antibody Name:
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP 2/0-Ag 14
Epitope Location or Sequence:
Antibody Registry ID: AB_528192
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DS1 was deposited to the DSHB by Poole, A.R. (DSHB Hybridoma Product DS1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.