DSAP47-1 (nc46)
Catalog Fields
Antigen: Synapse-associated protein 47kD
Available to For-Profits: Yes
Antigen Species: Drosophila
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1, kappa light chain
Depositor: Buchner, E. / Hofbauer, A.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Ant, Drosophila, Fish, Insects, Mosquito
Depositors Institution: University of Wuerzburg - Germany
Antigen Molecular Weight: 47 kDa
Depositors Notes: The antibody nc46 recognizes 9 SAP47 isoforms (see PMID: 15117418).
Immunogen: Brain homogenate
Gene: Sap47
Alternate Antibody Name:
Alternate Gene Names: Dmel_CG8884
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: P3X.63-Ag8.653
Epitope Mapped: Yes
Uniprot ID: A0A0B4LH71
Epitope Location or Sequence: N-terminal decapeptide FSGLTNQFTS
Entrez Gene ID: 41938
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_2617213
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
Additional Information: RRID:AB_2617213. FBgn0013334.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DSAP47-1 (nc46) was deposited to the DSHB by Buchner, E. / Hofbauer, A. (DSHB Hybridoma Product DSAP47-1 (nc46))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
22 References
Initial Publication
The sap47 gene of Drosophila melanogaster codes for a novel conserved neuronal protein associated with synaptic terminals.
Buchner E
Brain research. Molecular brain research 32.1 (1995 Aug): 45-54.