DSAP47-1 (nc46)

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SKU: DSAP47-1 (nc46)
View product citations for antibody DSAP47-1 (nc46) on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Synapse-associated protein 47kD
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Buchner, E. / Hofbauer, A.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Wuerzburg - Germany
Positive Tested Species Reactivity: Ant, Drosophila, Fish, Insects, Mosquito
Depositors Notes: The antibody nc46 recognizes 9 SAP47 isoforms (see PMID: 15117418).
Antigen Molecular Weight: 47 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Sap47
Immunogen: Brain homogenate
Alternate Gene Names: Dmel_CG8884
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X.63-Ag8.653
Epitope Location or Sequence: N-terminal decapeptide FSGLTNQFTS
Uniprot ID: A0A0B4LH71 
Immunogen Sequence: Full length protein
Entrez Gene ID: 41938 
Additional Characterization:  
Antibody Registry ID: AB_2617213 
Additional Information: RRID:AB_2617213. FBgn0013334.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DSAP47-1 (nc46) was deposited to the DSHB by Buchner, E. / Hofbauer, A. (DSHB Hybridoma Product DSAP47-1 (nc46))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

22 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    The sap47 gene of Drosophila melanogaster codes for a novel conserved neuronal protein associated with synaptic terminals.
    Buchner E
    Brain research. Molecular brain research 32.1 (1995 Aug): 45-54.

    Innervation of the ring gland of Drosophila melanogaster.
    Korge G
    The Journal of comparative neurology 431.4 (2001 Mar 19): 481-91.

    Behavioral and synaptic plasticity are impaired upon lack of the synaptic protein SAP47.
    Gerber B
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.9 (2011 Mar 2): 3508-18.

    Sra-1 interacts with Kette and Wasp and is required for neuronal and bristle development in Drosophila.
    Klämbt C
    Development (Cambridge, England) 131.16 (2004 Aug): 3981-9.

    The Drosophila microtubule associated protein Futsch is phosphorylated by Shaggy/Zeste-white 3 at an homologous GSK3beta phosphorylation site in MAP1B.
    Gordon-Weeks PR
    Molecular and cellular neurosciences 33.2 (2006 Oct): 188-99.

    Drosophila RSK negatively regulates bouton number at the neuromuscular junction.
    Sendtner M
    Developmental neurobiology 69.4 (2009 Mar): 212-20.

    The hangover gene negatively regulates bouton addition at the Drosophila neuromuscular junction.
    Scholz H
    Mechanisms of development 125.8 (2008 Aug): 700-11.

    Auditory Efferent System Modulates Mosquito Hearing.
    Göpfert MC
    Current biology : CB 26.15 (2016 Aug 8): 2028-2036.

    A multisensory centrifugal neuron in the olfactory pathway of heliothine moths.
    Berg BG
    The Journal of comparative neurology 521.1 (2013 Jan 1): 152-68.

    A multisensory centrifugal neuron in the olfactory pathway of heliothine moths.
    Berg BG
    The Journal of comparative neurology 521.1 (2013 Jan 1): 152-68.

    Differential parallel processing of olfactory information in the honeybee, Apis mellifera L.
    Menzel R
    Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology 188.5 (2002 Jun): 359-70.

    WB References

    Targeted mutagenesis of the Sap47 gene of Drosophila: flies lacking the synapse associated protein of 47 kDa are viable and fertile.
    Buchner E
    BMC neuroscience 5. (2004 Apr 29): 16.

    Behavioral and synaptic plasticity are impaired upon lack of the synaptic protein SAP47.
    Gerber B
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.9 (2011 Mar 2): 3508-18.

    Structure-function analysis of the cysteine string protein in Drosophila: cysteine string, linker and C terminus.
    Buchner E
    The Journal of experimental biology 207.Pt 8 (2004 Mar): 1323-34.

    The Drosophila melanogaster DmCK2beta transcription unit encodes for functionally non-redundant protein isoforms.
    Raabe T
    Gene 374. (2006 Jun 7): 142-52.

    The Drosophila p21 activated kinase Mbt regulates the actin cytoskeleton and adherens junctions to control photoreceptor cell morphogenesis.
    Raabe T
    Mechanisms of development 124.1 (2007 Jan): 78-90.

    Identification of Eps15 as antigen recognized by the monoclonal antibodies aa2 and ab52 of the Wuerzburg Hybridoma Library against Drosophila brain.
    Buchner E
    PloS one 6.12 (2011): e29352.

    Bruchpilot in ribbon-like axonal agglomerates, behavioral defects, and early death in SRPK79D kinase mutants of Drosophila.
    Buchner E
    PLoS genetics 5.10 (2009 Oct): e1000700.

    The relative role of the T-domain and flanking sequences for developmental control and transcriptional regulation in protein chimeras of Drosophila OMB and ORG-1.
    Pflugfelder GO
    Mechanisms of development 122.1 (2005 Jan): 81-96.

    Synapsin function in GABA-ergic interneurons is required for short-term olfactory habituation.
    Ramaswami M
    The Journal of neuroscience : the official journal of the Society for Neuroscience 33.42 (2013 Oct 16): 16576-85.

    The relative role of the T-domain and flanking sequences for developmental control and transcriptional regulation in protein chimeras of Drosophila OMB and ORG-1.
    Pflugfelder GO
    Mechanisms of development 122.1 (2005 Jan): 81-96.

    IHC References

    The sap47 gene of Drosophila melanogaster codes for a novel conserved neuronal protein associated with synaptic terminals.
    Buchner E
    Brain research. Molecular brain research 32.1 (1995 Aug): 45-54.

    Targeted mutagenesis of the Sap47 gene of Drosophila: flies lacking the synapse associated protein of 47 kDa are viable and fertile.
    Buchner E
    BMC neuroscience 5. (2004 Apr 29): 16.

    The Wuerzburg hybridoma library against Drosophila brain.
    Buchner E
    Journal of neurogenetics 23.1-2 (2009): 78-91.

    Drosophila Futsch/22C10 is a MAP1B-like protein required for dendritic and axonal development.
    Klämbt C
    Neuron 26.2 (2000 May): 357-70.

    Behavioral and synaptic plasticity are impaired upon lack of the synaptic protein SAP47.
    Gerber B
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.9 (2011 Mar 2): 3508-18.

    Sra-1 interacts with Kette and Wasp and is required for neuronal and bristle development in Drosophila.
    Klämbt C
    Development (Cambridge, England) 131.16 (2004 Aug): 3981-9.

    The control of mandible movements in the ant Odontomachus.
    Gronenberg W
    Journal of insect physiology 45.3 (1999 Mar): 231-240.

    Flies lacking all synapsins are unexpectedly healthy but are impaired in complex behaviour.
    Buchner E
    The European journal of neuroscience 20.3 (2004 Aug): 611-22.

    Epitope Map References

    The Wuerzburg hybridoma library against Drosophila brain.
    Buchner E
    Journal of neurogenetics 23.1-2 (2009): 78-91.

    All References

    The sap47 gene of Drosophila melanogaster codes for a novel conserved neuronal protein associated with synaptic terminals.
    Buchner E
    Brain research. Molecular brain research 32.1 (1995 Aug): 45-54.

    Targeted mutagenesis of the Sap47 gene of Drosophila: flies lacking the synapse associated protein of 47 kDa are viable and fertile.
    Buchner E
    BMC neuroscience 5. (2004 Apr 29): 16.

    The Wuerzburg hybridoma library against Drosophila brain.
    Buchner E
    Journal of neurogenetics 23.1-2 (2009): 78-91.

    Drosophila Futsch/22C10 is a MAP1B-like protein required for dendritic and axonal development.
    Klämbt C
    Neuron 26.2 (2000 May): 357-70.

    Behavioral and synaptic plasticity are impaired upon lack of the synaptic protein SAP47.
    Gerber B
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.9 (2011 Mar 2): 3508-18.

    Sra-1 interacts with Kette and Wasp and is required for neuronal and bristle development in Drosophila.
    Klämbt C
    Development (Cambridge, England) 131.16 (2004 Aug): 3981-9.

    The control of mandible movements in the ant Odontomachus.
    Gronenberg W
    Journal of insect physiology 45.3 (1999 Mar): 231-240.

    Flies lacking all synapsins are unexpectedly healthy but are impaired in complex behaviour.
    Buchner E
    The European journal of neuroscience 20.3 (2004 Aug): 611-22.

    Innervation of the ring gland of Drosophila melanogaster.
    Korge G
    The Journal of comparative neurology 431.4 (2001 Mar 19): 481-91.

    The Drosophila microtubule associated protein Futsch is phosphorylated by Shaggy/Zeste-white 3 at an homologous GSK3beta phosphorylation site in MAP1B.
    Gordon-Weeks PR
    Molecular and cellular neurosciences 33.2 (2006 Oct): 188-99.

    Drosophila RSK negatively regulates bouton number at the neuromuscular junction.
    Sendtner M
    Developmental neurobiology 69.4 (2009 Mar): 212-20.

    The hangover gene negatively regulates bouton addition at the Drosophila neuromuscular junction.
    Scholz H
    Mechanisms of development 125.8 (2008 Aug): 700-11.

    Auditory Efferent System Modulates Mosquito Hearing.
    Göpfert MC
    Current biology : CB 26.15 (2016 Aug 8): 2028-2036.

    A multisensory centrifugal neuron in the olfactory pathway of heliothine moths.
    Berg BG
    The Journal of comparative neurology 521.1 (2013 Jan 1): 152-68.

    Differential parallel processing of olfactory information in the honeybee, Apis mellifera L.
    Menzel R
    Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology 188.5 (2002 Jun): 359-70.

    Structure-function analysis of the cysteine string protein in Drosophila: cysteine string, linker and C terminus.
    Buchner E
    The Journal of experimental biology 207.Pt 8 (2004 Mar): 1323-34.

    The Drosophila melanogaster DmCK2beta transcription unit encodes for functionally non-redundant protein isoforms.
    Raabe T
    Gene 374. (2006 Jun 7): 142-52.

    The Drosophila p21 activated kinase Mbt regulates the actin cytoskeleton and adherens junctions to control photoreceptor cell morphogenesis.
    Raabe T
    Mechanisms of development 124.1 (2007 Jan): 78-90.

    Identification of Eps15 as antigen recognized by the monoclonal antibodies aa2 and ab52 of the Wuerzburg Hybridoma Library against Drosophila brain.
    Buchner E
    PloS one 6.12 (2011): e29352.

    Bruchpilot in ribbon-like axonal agglomerates, behavioral defects, and early death in SRPK79D kinase mutants of Drosophila.
    Buchner E
    PLoS genetics 5.10 (2009 Oct): e1000700.

    The relative role of the T-domain and flanking sequences for developmental control and transcriptional regulation in protein chimeras of Drosophila OMB and ORG-1.
    Pflugfelder GO
    Mechanisms of development 122.1 (2005 Jan): 81-96.

    Synapsin function in GABA-ergic interneurons is required for short-term olfactory habituation.
    Ramaswami M
    The Journal of neuroscience : the official journal of the Society for Neuroscience 33.42 (2013 Oct 16): 16576-85.

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