DSAP47-2 (nb200)

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$40.00
SKU: DSAP47-2 (nb200)
View product citations for antibody DSAP47-2 (nb200) on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Synapse-associated protein 47kD
Hybridoma Cells Available: Yes
Antigen Species: Drosophila
Depositor: Buchner, E. / Hofbauer, A.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Wuerzburg - Germany
Positive Tested Species Reactivity: Drosophila
Depositors Notes: This antibody stains the synaptic neuropil.
Antigen Molecular Weight: 47 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Sap47
Immunogen: Brain homogenate
Alternate Gene Names: Dmel_CG8884
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X.63-Ag8.653
Epitope Location or Sequence: Central domain QQAKHF
Uniprot ID: A0A0B4LH71 
Immunogen Sequence: Full length protein
Entrez Gene ID: 41938 
Additional Characterization:  
Antibody Registry ID: AB_10805877 
Additional Information: RRID:AB_10805877. FBgn0013334.
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
DSAP47-2 (nb200) was deposited to the DSHB by Buchner, E. / Hofbauer, A. (DSHB Hybridoma Product DSAP47-2 (nb200))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication
    WB References

    The Wuerzburg hybridoma library against Drosophila brain.
    Buchner E
    Journal of neurogenetics 23.1-2 (2009): 78-91.

    IHC References

    Differential parallel processing of olfactory information in the honeybee, Apis mellifera L.
    Menzel R
    Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology 188.5 (2002 Jun): 359-70.

    The Wuerzburg hybridoma library against Drosophila brain.
    Buchner E
    Journal of neurogenetics 23.1-2 (2009): 78-91.

    Epitope Map References

    The Wuerzburg hybridoma library against Drosophila brain.
    Buchner E
    Journal of neurogenetics 23.1-2 (2009): 78-91.

    All References

    Differential parallel processing of olfactory information in the honeybee, Apis mellifera L.
    Menzel R
    Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology 188.5 (2002 Jun): 359-70.

    The Wuerzburg hybridoma library against Drosophila brain.
    Buchner E
    Journal of neurogenetics 23.1-2 (2009): 78-91.

    The sap47 gene of Drosophila melanogaster codes for a novel conserved neuronal protein associated with synaptic terminals.
    Buchner E
    Brain research. Molecular brain research 32.1 (1995 Aug): 45-54.

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