Dcbl Ex6 8C4

(0) No Reviews yet
$45.00 to $425.00
SKU: Dcbl Ex6 8C4
View product citations for antibody Dcbl Ex6 8C4 on CiteAb

In Stock

Available: 42

DSHB Data Sheet

Catalog Fields

Antigen: D-cbl protein
Available to For-Profits: Yes
Antigen Species: Drosophila
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Schupbach, T.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Drosophila
Depositors Institution: Princeton University
Antigen Molecular Weight: 51.4 kDa
Depositors Notes:
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Recombinant 6X-His D-Cbl fusion protein
Gene: Cbl
Alternate Antibody Name:
Alternate Gene Names: Dmel_CG7037
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain:
Epitope Mapped: No
Uniprot ID: P91683 
Epitope Location or Sequence:
Entrez Gene ID: 38961 
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_10571936 
Additional Characterization:  
Recommended Applications: Immunofluorescence, Western Blot
Additional Information: RRID: AB_10571936 FBgn0020224.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Dcbl Ex6 8C4 was deposited to the DSHB by Schupbach, T. (DSHB Hybridoma Product Dcbl Ex6 8C4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication

    Differential effects of Cbl isoforms on Egfr signaling in Drosophila.
    Schüpbach T
    Mechanisms of development 123.6 (2006 Jun): 450-62.

    IF References

    Differential effects of Cbl isoforms on Egfr signaling in Drosophila.
    Schüpbach T
    Mechanisms of development 123.6 (2006 Jun): 450-62.

    WB References

    Differential effects of Cbl isoforms on Egfr signaling in Drosophila.
    Schüpbach T
    Mechanisms of development 123.6 (2006 Jun): 450-62.

    All References

    Differential effects of Cbl isoforms on Egfr signaling in Drosophila.
    Schüpbach T
    Mechanisms of development 123.6 (2006 Jun): 450-62.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review