Duct-1 (2.21B4)

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SKU: Duct-1 (2.21B4)

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DSHB Data Sheet

Catalog Fields

Product Name/ID: Duct-1 (2.21B4)
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: RIgM
Gene Symbol:
Antibody Registry ID: AB_531827 
Uniprot ID:  
RRID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: primary culture of pancreatic acinar cells
Clone:
Immunogen Sequence:
Myeloma Strain:
Epitope Mapped: No
Antigen Name: duct, exocrine gland
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 1/31/2001
Antigen Molecular Weight:
Depositor: De Lisle, R.C.
Antigen Sequence:
Depositor Institution: University of Kansas School of Medicine
Antigen Species: mouse
Depositor Notes: IHC note: apical plasma membrane of pancreatic, salivary, and liver duct cells; 4 % paraformaldehyde or fresh frozen/MeOH acetone
Host Species: rat
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Mouse
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Duct-1 (2.21B4) was deposited to the DSHB by De Lisle, R.C. (DSHB Hybridoma Product Duct-1 (2.21B4))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • Initial Publication
  • All References
  • Initial Publication

    Monoclonal antibodies as probes for plasma membrane domains in the exocrine pancreas.
    Williams JA
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 36.8 (1988 Aug): 1043-51.

    All References

    Monoclonal antibodies as probes for plasma membrane domains in the exocrine pancreas.
    Williams JA
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 36.8 (1988 Aug): 1043-51.

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