E/C8

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SKU: E/C8

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Available: 39

DSHB Data Sheet

Catalog Fields

Product Name/ID: E/C8
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgM
Gene Symbol:
Antibody Registry ID: AB_531792 
Uniprot ID:  
RRID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: Freshly dissected 7-day embryonic chicken sensory ganglia
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: SP 2/0
Epitope Mapped: No
Antigen Name: NAPA-73 (neurofilament-associated protein, 73 kDa)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 9/28/1988
Antigen Molecular Weight: Apparent: 73 kDa
Depositor: Ciment, G.
Antigen Sequence:
Depositor Institution: Oregon Health Sciences University
Antigen Species: chicken
Depositor Notes: Avian specific. This antibody does not appear to work for western blots.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Avian
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
E/C8 was deposited to the DSHB by Ciment, G. (DSHB Hybridoma Product E/C8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

13 References

  • Initial Publication
  • IF References
  • IHC References
  • IP References
  • All References
  • Initial Publication
    IF References
    IHC References
    IP References
    All References

    Early appearance in neural crest and crest-derived cells of an antigenic determinant present in avian neurons.
    Weston JA
    Developmental biology 93.2 (1982 Oct): 355-67.

    Close link between cutaneous nerve pattern development and feather morphogenesis demonstrated by experimental production of neo-apteria and ectopic feathers: implication of chondroitin sulphate proteoglycans and other matrix molecules.
    Saxod R
    Anatomy and embryology 195.5 (1997 May): 457-66.

    Characterization of HNK-1 antigens during the formation of the avian enteric nervous system.
    Meijers C
    Development (Cambridge, England) 115.2 (1992 Jun): 561-72.

    Transient expression of a ventricular myosin heavy chain isoform in developing chicken intrafusal muscle fibers.
    Maier A
    Histochemistry 99.4 (1993 Apr): 333-8.

    Developmental expression of a voltage-dependent potassium channel (Kv3.1) in auditory neurons without cochlear input.
    Morest DK
    Journal of neuroscience research 65.2 (2001 Jul 15): 121-8.

    A novel intermediate filament-associated protein, NAPA-73, that binds to different filament types at different stages of nervous system development.
    Weston JA
    The Journal of cell biology 102.1 (1986 Jan): 246-51.

    Basal lamina development in chicken muscle spindles.
    Mayne R
    Developmental dynamics : an official publication of the American Association of Anatomists 202.3 (1995 Mar): 284-93.

    Multipotent caudal neural progenitors derived from human pluripotent stem cells that give rise to lineages of the central and peripheral nervous system.
    Dottori M
    Stem cells (Dayton, Ohio) 33.6 (2015 Jun): 1759-70.

    Retinoic acid upregulates ret and induces chain migration and population expansion in vagal neural crest cells to colonise the embryonic gut.
    Newgreen DF
    PloS one 8.5 (2013): e64077.

    Reelin inhibits migration of sympathetic preganglionic neurons in the spinal cord of the chick.
    Yip JW
    The Journal of comparative neurology 519.10 (2011 Jul 1): 1970-8.

    Axon contacts and acetylcholinesterase activity on chicken intrafusal muscle fiber types identified by their myosin heavy chain composition.
    Maier A
    Anatomy and embryology 184.5 (1991): 497-505.

    Development of chicken intrafusal muscle fibers.
    Maier A
    Cell and tissue research 274.2 (1993 Nov): 383-91.

    Transient expression of GAP-43 in nonneuronal cells of the embryonic chicken limb.
    Ciment G
    Developmental biology 149.2 (1992 Feb): 406-14.

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