E/C8
Catalog Fields
Antigen: NAPA-73 (neurofilament-associated protein, 73 kDa)
Available to For-Profits: Yes
Antigen Species: chicken
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgM
Depositor: Ciment, G.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Avian
Depositors Institution: Oregon Health Sciences University
Antigen Molecular Weight: Apparent: 73 kDa
Depositors Notes: Avian specific. This antibody does not appear to work for western blots.
Immunogen: Freshly dissected 7-day embryonic chicken sensory ganglia
Gene:
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: SP 2/0
Epitope Mapped: No
Antibody Registry ID: AB_531792
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
E/C8 was deposited to the DSHB by Ciment, G. (DSHB Hybridoma Product E/C8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
13 References
Initial Publication
Early appearance in neural crest and crest-derived cells of an antigenic determinant present in avian neurons.
Weston JA
Developmental biology 93.2 (1982 Oct): 355-67.