E1.9

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SKU: E1.9

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DSHB Data Sheet

Catalog Fields

Product Name/ID: E1.9
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name:
Ab Isotype: MIgM
Gene Symbol:
Antibody Registry ID: AB_531876 
Uniprot ID:  
RRID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: Whole murine cells from the mid- and hindbrain (development stage: E12)
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: FOX-NY
Epitope Mapped: No
Antigen Name: neurofilaments, primary sensory and motor neurons
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 10/24/1990
Antigen Molecular Weight:
Depositor: Gilbert, W. / Stainier, D.
Antigen Sequence:
Depositor Institution: Max Planck Institute for Heart & Lung Research
Antigen Species: Mouse
Depositor Notes: filaments in growing primary sensory and motor neurons
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Mouse, Rat
Additional Information: In the original paper for E1.9 [PMID: 2300585], the immunogen was described as rat E12, but the subsequent paper from the same authors and in the Depositor's notes it was described as mouse E11. The E1.9 epitope is developmentally regulated. E1.9 recognizes a cytoplasmic epitope in the primary sensory and motor neurons during axonal outgrowth, appearing at E8.5 and disappearing by E12 in the mouse when the same cell populations become positive for mAb B10. It stains neural crest-derived sensory neurons (e.g., neurons of dorsal root ganglia) as well as placode-derived sensory neurons (e.g.neurons of cranial nerves VII and VIII). It is not clear which filaments are recognized by this antibody. For this reason we didn't link it to a Uniprot or gene ID. See mAb B10 for a related antibody.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
E1.9 was deposited to the DSHB by Gilbert, W. / Stainier, D. (DSHB Hybridoma Product E1.9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

4 References

  • Initial Publication
  • IF References
  • All References
  • Initial Publication

    Pioneer neurons in the mouse trigeminal sensory system.
    Gilbert W
    Proceedings of the National Academy of Sciences of the United States of America 87.3 (1990 Feb): 923-7.

    IF References

    Pioneer neurons in the mouse trigeminal sensory system.
    Gilbert W
    Proceedings of the National Academy of Sciences of the United States of America 87.3 (1990 Feb): 923-7.

    Neuronal differentiation and maturation in the mouse trigeminal sensory system, in vivo and in vitro.
    Gilbert W
    The Journal of comparative neurology 311.2 (1991 Sep 8): 300-12.

    Expandable and Rapidly Differentiating Human Induced Neural Stem Cell Lines for Multiple Tissue Engineering Applications.
    Kaplan DL
    Stem cell reports 7.3 (2016 Sep 13): 557-570.

    All References

    Pioneer neurons in the mouse trigeminal sensory system.
    Gilbert W
    Proceedings of the National Academy of Sciences of the United States of America 87.3 (1990 Feb): 923-7.

    Neuronal differentiation and maturation in the mouse trigeminal sensory system, in vivo and in vitro.
    Gilbert W
    The Journal of comparative neurology 311.2 (1991 Sep 8): 300-12.

    Expandable and Rapidly Differentiating Human Induced Neural Stem Cell Lines for Multiple Tissue Engineering Applications.
    Kaplan DL
    Stem cell reports 7.3 (2016 Sep 13): 557-570.

    Differentiation of mammalian vestibular hair cells from conditionally immortal, postnatal supporting cells.
    Holley MC
    The Journal of neuroscience : the official journal of the Society for Neuroscience 19.21 (1999 Nov 1): 9445-58.

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