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$45.00 to $425.00
SKU: F2-P3E3
View product citations for antibody F2-P3E3 on CiteAb

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Available: 14

DSHB Data Sheet

Catalog Fields

Antigen: Apoptotic marker, intestinal phagocytes
Available to For-Profits: Yes
Antigen Species: C. elegans
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgM, kappa light chain
Depositor: Hermann, G.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: A. ellesmerensis, A. nanus, C. briggsae, C. elegans, R. axie
Depositors Institution: Lewis and Clark College, Portland, OR
Antigen Molecular Weight:
Depositors Notes: The target antigen F2-P3E3 has not yet been identified.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: 5hr fixed embryo lysate
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: Fox NY
Epitope Mapped: No
Uniprot ID:  
Epitope Location or Sequence:
Entrez Gene ID:  
Immunogen Sequence: Full length sequence
Antibody Registry ID: AB_760347 
Additional Characterization:  
Recommended Applications: Immunofluorescence
Additional Information: RRID:AB_760347
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
F2-P3E3 was deposited to the DSHB by Hermann, G. (DSHB Hybridoma Product F2-P3E3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

3 References

  • Initial Publication
  • IF References
  • All References
  • Initial Publication

    Characterization of a conserved apoptotic marker expressed in Caenorhabditis elegans phagocytic cells.
    Hermann GJ
    Biochemical and biophysical research communications 335.4 (2005 Oct 7): 1231-8.

    IF References
    All References

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