F2-P3E3

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$40.00
SKU: F2-P3E3
View product citations for antibody F2-P3E3 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Apoptotic marker, intestinal phagocytes
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Hermann, G.
Isotype: MIgM, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Lewis and Clark College, Portland, OR
Positive Tested Species Reactivity: A. ellesmerensis, A. nanus, C. briggsae, C. elegans, R. axie
Depositors Notes: The target antigen F2-P3E3 has not yet been identified.
Antigen Molecular Weight:
Human Protein Atlas:
Predicted Species Reactivity:  
Gene:
Immunogen: 5hr fixed embryo lysate
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: Fox NY
Epitope Location or Sequence:
Uniprot ID:
Immunogen Sequence: Full length sequence
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_760347 
Additional Information: RRID:AB_760347
Recommended Applications: Immunofluorescence
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
F2-P3E3 was deposited to the DSHB by Hermann, G. (DSHB Hybridoma Product F2-P3E3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • IF References
  • All References
  • Initial Publication

    Characterization of a conserved apoptotic marker expressed in Caenorhabditis elegans phagocytic cells.
    Hermann GJ
    Biochemical and biophysical research communications 335.4 (2005 Oct 7): 1231-8.

    IF References
    All References

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