F30
Catalog Fields
Antigen: Myosin heavy chain, avian fast isoforms and rat, rabbit, and turtle isoforms
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Stockdale, F.E.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Stanford University School of Medicine
Positive Tested Species Reactivity:
Depositors Notes: Specificity note: reacts with a number of species. Characterization: specific for isoforms of myosin heavy chain associated with fast contracting muscle; recognizes multiple fast isoforms including isoforms present in avian and mammalian species. IHC note: works well on Western blots and immunostaining of tissue sections.
Predicted Species Reactivity:
Gene:
Immunogen: myosin isolated from chicken pectoralis major muscle
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3/NS1.1Ag4-1
Epitope Location or Sequence:
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
F30 was deposited to the DSHB by Stockdale, F.E. (DSHB Hybridoma Product F30)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.
1 Reference
Initial Publication
Slow and fast myosin heavy chain content defines three types of myotubes in early muscle cell cultures.
Stockdale FE
The Journal of cell biology 101.5 Pt 1 (1985 Nov): 1643-50.