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SKU: FE-A5-s
View product citations for antibody FE-A5 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Embryonal carcinoma cell surface carbohydrate
Hybridoma Cells Available: No
Antigen Species: Rat
Depositor: Fenderson, B. / Eddy, M.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: Thomas Jefferson University
Positive Tested Species Reactivity: Fish, Mammal, Mouse, Ovine, Rat
Depositors Notes: This antibody recognizes a carbohydrate epitope found on glycolipids and glycoproteins: Gal b 1-4 G1cNAc b 1-3 R. This carbohydrate moiety is found on the surface of mouse NULLI-SCC1 and F9 testis embryonal carcinoma cells. Bind of FE-A5 is strongly inhibited by the presence of N-acetyllactoseamine, lactose and D-Galactose.
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Immunogen: Rat testicular cell lysate
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence: Carbohydrate moiety Gal b 1-4 G1cNAc b 1-3 R
Uniprot ID:  
Immunogen Sequence: Full length protein
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_531818 
Additional Information: FE-A5 has been successfully used in TLC overlay applications (PMID: 26405105).
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
FE-A5 was deposited to the DSHB by Fenderson, B. / Eddy, M. (DSHB Hybridoma Product FE-A5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

8 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • FFPE References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References
    ELISA References
    FFPE References
    Epitope Map References
    All References

    Immunohistochemical localization of two monoclonal antibody-defined carbohydrate antigens during early murine embryogenesis.
    Eddy EM
    Developmental biology 100.2 (1983 Dec): 318-27.

    Localization and function of ATP and GABAA receptors expressed by nociceptors and other postnatal sensory neurons in rat.
    MacDermott AB
    The Journal of physiology 549.Pt 1 (2003 May 15): 131-42.

    Stage-specific expression of three cell surface carbohydrate antigens during murine spermatogenesis detected with monoclonal antibodies.
    Eddy EM
    Developmental biology 103.1 (1984 May): 117-28.

    LAT region factors mediating differential neuronal tropism of HSV-1 and HSV-2 do not act in trans.
    Margolis TP
    PloS one 7.12 (2012): e53281.

    A5-positive primary sensory neurons are nonpermissive for productive infection with herpes simplex virus 1 in vitro.
    Margolis TP
    Journal of virology 85.13 (2011 Jul): 6669-77.

    Latency-associated transcript (LAT) exon 1 controls herpes simplex virus species-specific phenotypes: reactivation in the guinea pig genital model and neuron subtype-specific latent expression of LAT.
    Krause PR
    Journal of virology 83.19 (2009 Oct): 10007-15.

    Monoclonal antibody directed to Le(y) oligosaccharide inhibits implantation in the mouse.
    Fenderson BA
    Biology of reproduction 52.4 (1995 Apr): 903-12.

    A monoclonal antibody defining a binary N-acetyllactosaminyl structure in lactoisooctaosylceramide (IV6Gal beta 1----4GlcNAcnLc6): a useful probe for determining differential glycosylation patterns between normal and transformed human fibroblasts.
    Hakomori SI
    Molecular immunology 23.7 (1986 Jul): 747-54.

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