Catalog Fields
Antigen: Embryonal carcinoma cell surface carbohydrate
Hybridoma Cells Available: No
Antigen Species: Rat
Depositor: Fenderson, B. / Eddy, M.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: Thomas Jefferson University
Positive Tested Species Reactivity: Fish, Mammal, Mouse, Ovine, Rat
Depositors Notes: This antibody recognizes a carbohydrate epitope found on glycolipids and glycoproteins: Gal b 1-4 G1cNAc b 1-3 R. This carbohydrate moiety is found on the surface of mouse NULLI-SCC1 and F9 testis embryonal carcinoma cells. Bind of FE-A5 is strongly inhibited by the presence of N-acetyllactoseamine, lactose and D-Galactose.
Predicted Species Reactivity:
Gene:
Immunogen: Rat testicular cell lysate
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence: Carbohydrate moiety Gal b 1-4 G1cNAc b 1-3 R
Antibody Registry ID: AB_531818
Additional Information: FE-A5 has been successfully used in TLC overlay applications (PMID: 26405105).
Recommended Applications: FFPE, Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
FE-A5 was deposited to the DSHB by Fenderson, B. / Eddy, M. (DSHB Hybridoma Product FE-A5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
8 References
Initial Publication
Immunohistochemical localization of two monoclonal antibody-defined carbohydrate antigens during early murine embryogenesis.
Eddy EM
Developmental biology 100.2 (1983 Dec): 318-27.